Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan

Zhenqing Zhang; Jin Xie; Jian Liu; Robert J. Linhardt

doi:10.1016/j.jasms.2007.10.012

Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan

Zhenqing Zhang, Jin Xie, Jian Liu, Robert J. Linhardt

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19 Scopus citations

Abstract

Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1→3 and in NAH 1→4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions ^0,2A_n and ^0,2A_n-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an ^0,2A₂ ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.

Original language	English
Pages (from-to)	82-90
Number of pages	9
Journal	Journal of the American Society for Mass Spectrometry
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/j.jasms.2007.10.012
State	Published - Jan 2008
Externally published	Yes

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title = "Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan",

abstract = "Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1→3 and in NAH 1→4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.",

author = "Zhenqing Zhang and Jin Xie and Jian Liu and Linhardt, {Robert J.}",

note = "Funding Information: The authors thank Tatiana Laremore for her careful reading of the manuscript and the NIH for supporting this research through grants GM38060 and HL62244.",

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doi = "10.1016/j.jasms.2007.10.012",

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T1 - Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan

AU - Zhang, Zhenqing

AU - Xie, Jin

AU - Liu, Jian

AU - Linhardt, Robert J.

N1 - Funding Information: The authors thank Tatiana Laremore for her careful reading of the manuscript and the NIH for supporting this research through grants GM38060 and HL62244.

PY - 2008/1

Y1 - 2008/1

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AB - Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1→3 and in NAH 1→4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.

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Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan

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