T cell receptor Vβ gene utilization in myelin basic protein specific clones from CXJ1 recombinant inbred mice

Bernadette Kalman; Robert L. Knobler; Fred D. Lublin

doi:10.1016/0165-5728(93)90091-C

T cell receptor Vβ gene utilization in myelin basic protein specific clones from CXJ1 recombinant inbred mice

Bernadette Kalman, Robert L. Knobler, Fred D. Lublin

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

CXJ1 mice are a recombinant inbred strain generated from experimental allergic encephalomyelitis (EAE) resistant BALB/c and EAE susceptible SJL/J progenitors. CXJ1 derive their major histocompatibility complex (MHC) class II and TCR genes from the BALB/c progenitor. However, their susceptibility to EAE is similar to SJL/J. Utilizing myelin basic protein (MBP)-specific CD4⁺ hybridoma clones and a MBP-specific T cell line (TCL) from CXJ1, we found the predominant T cell receptor (TCR) Vβ chain expression to be Vβ8 and Vβ13. Our data support the concept of preferential, but not exclusive, TCR Vβ usage in the MBP-specific response which is independent of MHC class II haplotype or immunodominant peptide.

Original language	English
Pages (from-to)	191-194
Number of pages	4
Journal	Journal of Neuroimmunology
Volume	43
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-5728(93)90091-C
State	Published - Mar 1993
Externally published	Yes

Keywords

Experimental allergic encephalomyelitis
Recombinant inbred mice
T cell receptor

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10.1016/0165-5728(93)90091-C

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@article{5eafd0e05d574d9c98b43daeb7cede0f,

title = "T cell receptor Vβ gene utilization in myelin basic protein specific clones from CXJ1 recombinant inbred mice",

abstract = "CXJ1 mice are a recombinant inbred strain generated from experimental allergic encephalomyelitis (EAE) resistant BALB/c and EAE susceptible SJL/J progenitors. CXJ1 derive their major histocompatibility complex (MHC) class II and TCR genes from the BALB/c progenitor. However, their susceptibility to EAE is similar to SJL/J. Utilizing myelin basic protein (MBP)-specific CD4+ hybridoma clones and a MBP-specific T cell line (TCL) from CXJ1, we found the predominant T cell receptor (TCR) Vβ chain expression to be Vβ8 and Vβ13. Our data support the concept of preferential, but not exclusive, TCR Vβ usage in the MBP-specific response which is independent of MHC class II haplotype or immunodominant peptide.",

keywords = "Experimental allergic encephalomyelitis, Recombinant inbred mice, T cell receptor",

author = "Bernadette Kalman and Knobler, {Robert L.} and Lublin, {Fred D.}",

note = "Funding Information: The technical assistance of M. Perreault is greatly appreciated. This work was supported in part by Research Grant RG-1722 from the National Multiple Sclerosis Society and NS-22145 from the National Institutes of Health.",

year = "1993",

month = mar,

doi = "10.1016/0165-5728(93)90091-C",

language = "English",

volume = "43",

pages = "191--194",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - T cell receptor Vβ gene utilization in myelin basic protein specific clones from CXJ1 recombinant inbred mice

AU - Kalman, Bernadette

AU - Knobler, Robert L.

AU - Lublin, Fred D.

N1 - Funding Information: The technical assistance of M. Perreault is greatly appreciated. This work was supported in part by Research Grant RG-1722 from the National Multiple Sclerosis Society and NS-22145 from the National Institutes of Health.

PY - 1993/3

Y1 - 1993/3

N2 - CXJ1 mice are a recombinant inbred strain generated from experimental allergic encephalomyelitis (EAE) resistant BALB/c and EAE susceptible SJL/J progenitors. CXJ1 derive their major histocompatibility complex (MHC) class II and TCR genes from the BALB/c progenitor. However, their susceptibility to EAE is similar to SJL/J. Utilizing myelin basic protein (MBP)-specific CD4+ hybridoma clones and a MBP-specific T cell line (TCL) from CXJ1, we found the predominant T cell receptor (TCR) Vβ chain expression to be Vβ8 and Vβ13. Our data support the concept of preferential, but not exclusive, TCR Vβ usage in the MBP-specific response which is independent of MHC class II haplotype or immunodominant peptide.

AB - CXJ1 mice are a recombinant inbred strain generated from experimental allergic encephalomyelitis (EAE) resistant BALB/c and EAE susceptible SJL/J progenitors. CXJ1 derive their major histocompatibility complex (MHC) class II and TCR genes from the BALB/c progenitor. However, their susceptibility to EAE is similar to SJL/J. Utilizing myelin basic protein (MBP)-specific CD4+ hybridoma clones and a MBP-specific T cell line (TCL) from CXJ1, we found the predominant T cell receptor (TCR) Vβ chain expression to be Vβ8 and Vβ13. Our data support the concept of preferential, but not exclusive, TCR Vβ usage in the MBP-specific response which is independent of MHC class II haplotype or immunodominant peptide.

KW - Experimental allergic encephalomyelitis

KW - Recombinant inbred mice

KW - T cell receptor

UR - http://www.scopus.com/inward/record.url?scp=0027458896&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(93)90091-C

DO - 10.1016/0165-5728(93)90091-C

M3 - Article

C2 - 7681448

AN - SCOPUS:0027458896

SN - 0165-5728

VL - 43

SP - 191

EP - 194

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

T cell receptor Vβ gene utilization in myelin basic protein specific clones from CXJ1 recombinant inbred mice

Abstract

Keywords

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