T cell receptor repertoire sequencing

Huixin Lin; Yonggang Peng; Xiangbin Chen; Yuebin Liang; Geng Tian; Jialiang Yang

doi:10.1007/978-1-0716-0904-0_1

T cell receptor repertoire sequencing

Huixin Lin, Yonggang Peng, Xiangbin Chen, Yuebin Liang, Geng Tian, Jialiang Yang

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and other medical fields. High-throughput sequencing promotes the studying in TCR repertoire. The chapter focuses on the whole process of TCR profiling, including DNA extraction, library construction, high-throughput sequencing, and how to analyze data.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	3-12
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-0716-0904-0_1
State	Published - 2020
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2204
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Data analysis
High-throughput sequencing
Library construction
T cell receptor repertoire

Access to Document

10.1007/978-1-0716-0904-0_1

Cite this

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abstract = "The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and other medical fields. High-throughput sequencing promotes the studying in TCR repertoire. The chapter focuses on the whole process of TCR profiling, including DNA extraction, library construction, high-throughput sequencing, and how to analyze data.",

keywords = "Data analysis, High-throughput sequencing, Library construction, T cell receptor repertoire",

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AU - Lin, Huixin

AU - Peng, Yonggang

AU - Chen, Xiangbin

AU - Liang, Yuebin

AU - Tian, Geng

AU - Yang, Jialiang

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.

PY - 2020

Y1 - 2020

N2 - The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and other medical fields. High-throughput sequencing promotes the studying in TCR repertoire. The chapter focuses on the whole process of TCR profiling, including DNA extraction, library construction, high-throughput sequencing, and how to analyze data.

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KW - Data analysis

KW - High-throughput sequencing

KW - Library construction

KW - T cell receptor repertoire

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ER -

T cell receptor repertoire sequencing

Abstract

Publication series

Keywords

Access to Document

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Cite this