T-cell-dependent hybrid resistance against a natural killer-resistant moloney virus-induced lymphoma (YWA): In vitro generation of cytotoxic lymphocytes

Fernando Merino; Alvar Grönberg; George Klein

doi:10.1016/0008-8749(84)90310-1

T-cell-dependent hybrid resistance against a natural killer-resistant moloney virus-induced lymphoma (YWA): In vitro generation of cytotoxic lymphocytes

Fernando Merino, Alvar Grönberg, George Klein

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A Moloney leukemia virus-induced lymphoma of the A.SW strain, YWA, was used to generate cytotoxic cells in vitro. Cocultivation of spleen cells from in vivo primed syngeneic and semisyngeneic mice with X-irradiated YWA tumor cells for 5 days resulted in a strong killing activity against YWA. The cytotoxicity was H-2 restricted and mediated by Thy-1.2-positive lymphocytes. f₁ hybrids with variable degrees of natural resistance to the YWA tumor in vivo all generated cytotoxic cells after secondary stimulation in vitro but showed differences in optimal responder:stimulator requirements.

Original language	English
Pages (from-to)	313-320
Number of pages	8
Journal	Cellular Immunology
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(84)90310-1
State	Published - Feb 1984
Externally published	Yes

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title = "T-cell-dependent hybrid resistance against a natural killer-resistant moloney virus-induced lymphoma (YWA): In vitro generation of cytotoxic lymphocytes",

abstract = "A Moloney leukemia virus-induced lymphoma of the A.SW strain, YWA, was used to generate cytotoxic cells in vitro. Cocultivation of spleen cells from in vivo primed syngeneic and semisyngeneic mice with X-irradiated YWA tumor cells for 5 days resulted in a strong killing activity against YWA. The cytotoxicity was H-2 restricted and mediated by Thy-1.2-positive lymphocytes. f1 hybrids with variable degrees of natural resistance to the YWA tumor in vivo all generated cytotoxic cells after secondary stimulation in vitro but showed differences in optimal responder:stimulator requirements.",

author = "Fernando Merino and Alvar Gr{\"o}nberg and George Klein",

note = "Funding Information: This investigationw as supportedb y PHS Grants CA 26782a nd 5 ROI CA 14054-08a, wardedb y the National CancerI nstitute,D HHS, and by the SwedishC ancerS ociety.F ernandoM erino is supportedb y a fellowshipf rom CancerR esearchI nstitute,N ew York.",

year = "1984",

month = feb,

doi = "10.1016/0008-8749(84)90310-1",

language = "English",

volume = "83",

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journal = "Cellular Immunology",

issn = "0008-8749",

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T1 - T-cell-dependent hybrid resistance against a natural killer-resistant moloney virus-induced lymphoma (YWA)

T2 - In vitro generation of cytotoxic lymphocytes

AU - Merino, Fernando

AU - Grönberg, Alvar

AU - Klein, George

N1 - Funding Information: This investigationw as supportedb y PHS Grants CA 26782a nd 5 ROI CA 14054-08a, wardedb y the National CancerI nstitute,D HHS, and by the SwedishC ancerS ociety.F ernandoM erino is supportedb y a fellowshipf rom CancerR esearchI nstitute,N ew York.

PY - 1984/2

Y1 - 1984/2

N2 - A Moloney leukemia virus-induced lymphoma of the A.SW strain, YWA, was used to generate cytotoxic cells in vitro. Cocultivation of spleen cells from in vivo primed syngeneic and semisyngeneic mice with X-irradiated YWA tumor cells for 5 days resulted in a strong killing activity against YWA. The cytotoxicity was H-2 restricted and mediated by Thy-1.2-positive lymphocytes. f1 hybrids with variable degrees of natural resistance to the YWA tumor in vivo all generated cytotoxic cells after secondary stimulation in vitro but showed differences in optimal responder:stimulator requirements.

AB - A Moloney leukemia virus-induced lymphoma of the A.SW strain, YWA, was used to generate cytotoxic cells in vitro. Cocultivation of spleen cells from in vivo primed syngeneic and semisyngeneic mice with X-irradiated YWA tumor cells for 5 days resulted in a strong killing activity against YWA. The cytotoxicity was H-2 restricted and mediated by Thy-1.2-positive lymphocytes. f1 hybrids with variable degrees of natural resistance to the YWA tumor in vivo all generated cytotoxic cells after secondary stimulation in vitro but showed differences in optimal responder:stimulator requirements.

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DO - 10.1016/0008-8749(84)90310-1

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JO - Cellular Immunology

JF - Cellular Immunology

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ER -

T-cell-dependent hybrid resistance against a natural killer-resistant moloney virus-induced lymphoma (YWA): In vitro generation of cytotoxic lymphocytes

Abstract

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