TY - JOUR
T1 - Systems scale interactive exploration reveals quantitative and qualitative differences in response to influenza and pneumococcal vaccines
AU - Obermoser, Gerlinde
AU - Presnell, Scott
AU - Domico, Kelly
AU - Xu, Hui
AU - Wang, Yuanyuan
AU - Anguiano, Esperanza
AU - Thompson-Snipes, Lu Ann
AU - Ranganathan, Rajaram
AU - Zeitner, Brad
AU - Bjork, Anna
AU - Anderson, David
AU - Speake, Cate
AU - Ruchaud, Emily
AU - Skinner, Jason
AU - Alsina, Laia
AU - Sharma, Mamta
AU - Dutartre, Helene
AU - Cepika, Alma
AU - Israelsson, Elisabeth
AU - Nguyen, Phuong
AU - Nguyen, Quynh Anh
AU - Harrod, A. Carson
AU - Zurawski, Sandra M.
AU - Pascual, Virginia
AU - Ueno, Hideki
AU - Nepom, Gerald T.
AU - Quinn, Charlie
AU - Blankenship, Derek
AU - Palucka, Karolina
AU - Banchereau, Jacques
AU - Chaussabel, Damien
N1 - Funding Information:
We thank our volunteer participants and all of the members of BIIR who assisted in this work. We thank I. Berthier, C. Boudreaux, A. Cobb, M. Hughes, B. Lemoine, I. Munagala, D. Nattamai, and colleagues for providing technical assistance with sample preparation, microarray processing, and flow cytometry. We thank A. Garcia-Sastre and R. Albrecht, Department of Microbiology, Mount Sinai School of Medicine, for advice and performing serological assays. We thank E. De Vol, N. Baldwin, and E. Whalen for advice and support in biostatistics and bioinformatics, G. Hayward for help depositing microarray data, M. Roy and O. Vargas for help with editing the wiki site, C. Samuelsen for organizational support, and R. Coffman for discussion of the manuscript. G.O. was supported by an Erwin Schrodinger Research Grant of the Austrian Science Fund and the Medical Research Fund MFF Tirol. The work of D.C., J.B., and K.P. are supported by the Baylor Health Care System Foundation and the National Institutes of Health (U19 AI08998, U19 AI057234, U01 AI082110, N01-AI-15416, and P01 CA084512). J.B., K.P., and D.C. designed the study; G.O., Y.W., E.A., L.T.-S., R.R., E.R, J.S., L.A., M.S., H.D., P.N., Q.-A.N., and S.M.Z. assisted in experiments; H.U. and V.P. supervised the experiments; A.C.H. recruited volunteers; G.O., H.X., Y.W., E.A., A.C., and D.B. analyzed the data; S.P., K.D., B.Z., A.B., D.A., C.S., E.I., G.T.N., and C.Q. provided bioinformatic tools and support, and G.O, K.P., and D.C prepared the manuscript.
PY - 2013/4/18
Y1 - 2013/4/18
N2 - Systems immunology approaches were employed to investigate innate and adaptive immune responses to influenza and pneumococcal vaccines. These two non-live vaccines show different magnitudes of transcriptional responses at different time points after vaccination. Software solutions were developed to explore correlates of vaccine efficacy measured as antibody titers at day 28. These enabled a further dissection of transcriptional responses. Thus, the innate response, measured within hours in the peripheral blood, was dominated by an interferon transcriptional signature after influenza vaccination and by an inflammation signature after pneumococcal vaccination. Day 7 plasmablast responses induced by both vaccines was more pronounced after pneumococcal vaccination. Together, these results suggest that comparing global immune responses elicited by different vaccines will be critical to our understanding of the immune mechanisms underpinning successful vaccination.
AB - Systems immunology approaches were employed to investigate innate and adaptive immune responses to influenza and pneumococcal vaccines. These two non-live vaccines show different magnitudes of transcriptional responses at different time points after vaccination. Software solutions were developed to explore correlates of vaccine efficacy measured as antibody titers at day 28. These enabled a further dissection of transcriptional responses. Thus, the innate response, measured within hours in the peripheral blood, was dominated by an interferon transcriptional signature after influenza vaccination and by an inflammation signature after pneumococcal vaccination. Day 7 plasmablast responses induced by both vaccines was more pronounced after pneumococcal vaccination. Together, these results suggest that comparing global immune responses elicited by different vaccines will be critical to our understanding of the immune mechanisms underpinning successful vaccination.
UR - http://www.scopus.com/inward/record.url?scp=84876745847&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2012.12.008
DO - 10.1016/j.immuni.2012.12.008
M3 - Article
C2 - 23601689
AN - SCOPUS:84876745847
SN - 1074-7613
VL - 38
SP - 831
EP - 844
JO - Immunity
JF - Immunity
IS - 4
ER -