The authors have developed a system in which a series of nine chromatin-associated DNA endonucleases have been separated from human lymphoblastoid cells. Different DNA endonuclease activities show selective activity on DNA containing one of a number of different types of adducts. Some of these damage-specific DNA endonuclease activities are absent or altered in cell types known to have defective DNA repair mechanisms. One such example is the genetic disease, xeroderma pigmentosum. Certain of these DNA endonuclease activities show a two- to threefold enhancement when the damaged DNA is in the form of nucleosomes, rather than as purified DNA, and this enhancement is absent in xeroderma pigmentosum, complementation group A. The results emphasize the important role nucleosome structure has on manipulating the interaction of these endonucleases with DNA.
|Number of pages||2|
|State||Published - Nov 1988|
|Event||Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society - New Orleans, LA, USA|
Duration: 4 Nov 1988 → 7 Nov 1988
|Conference||Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society|
|City||New Orleans, LA, USA|
|Period||4/11/88 → 7/11/88|