Synthetic peptides entrapped in microparticles can elicit cytotoxic T cell activity

Douglas F. Nixon, Catarina Hioe, Pei De Chen, Zuning Bian, Peter Kuebler, Ming Lie Li, Howard Qiu, Xuan Mao Li, Manmohan Singh, Julie Richardson, Paul McGee, Tim Zamb, Wayne Koff, Chang Yi Wang, Derek O'Hagan

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

Peptides from Plasmodium berghei circumsporozoite protein (CS) and influenza A virus nucleoprotein (NP) were entrapped in microparticles prepared from poly (lactide-colide polymers, and the microparticles were administered parenterally to mice. After immunization with single or multiple doses, splenocytes were tested for a cytotoxic T cell (CTL) response and high levels of CTL activity were detected. The CTL induced were CD8+, MHC class I restricted and could recognize virus infected cells. Peptide entrapped in microparticles of mean size <500 nm were better inducers of CTL than larger microparticles (mean >2 μm and above). Microparticles could also be used to deliver lipid modified peptides (lipopeptides) and elicited higher levels of cytolytic activity than either free peptide in microparticles or lipopeptide alone. Microparticles provide a novel way of inducing a CTL response using synthetic peptides.

Original languageEnglish
Pages (from-to)1523-1530
Number of pages8
JournalVaccine
Volume14
Issue number16
DOIs
StatePublished - Nov 1996
Externally publishedYes

Keywords

  • CTL
  • Lipopeptides
  • Microparticles
  • Peptides
  • Vaccines

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