TY - JOUR
T1 - Synthetic matrix enhances transplanted satellite cell engraftment in dystrophic and aged skeletal muscle with comorbid trauma
AU - Han, Woojin M.
AU - Anderson, Shannon E.
AU - Mohiuddin, Mahir
AU - Barros, Daniela
AU - Nakhai, Shadi A.
AU - Shin, Eunjung
AU - Amaral, Isabel Freitas
AU - Pêgo, Ana Paula
AU - García, Andrés J.
AU - Jang, Young C.
N1 - Publisher Copyright:
Copyright © 2018 The Authors.
PY - 2018/8/15
Y1 - 2018/8/15
N2 - Muscle satellite cells (MuSCs) play a central role in muscle regeneration, but their quantity and function decline with comorbidity of trauma, aging, and muscle diseases. Although transplantation of MuSCs in traumatically injured muscle in the comorbid context of aging or pathology is a strategy to boost muscle regeneration, an effective cell delivery strategy in these contexts has not been developed. We engineered a synthetic hydrogel-based matrix with optimal mechanical, cell-adhesive, and protease-degradable properties that promotes MuSC survival, proliferation, and differentiation. Furthermore, we establish a biomaterial-mediated cell delivery strategy for treating muscle trauma, where intramuscular injections may not be applicable. Delivery of MuSCs in the engineered matrix significantly improved in vivo cell survival, proliferation, and engraftment in nonirradiated and immunocompetent muscles of aged and dystrophic mice compared to collagen gels and cell-only controls. This platform may be suitable for treating craniofacial and limb muscle trauma, as well as postoperative wounds of elderly and dystrophic patients.
AB - Muscle satellite cells (MuSCs) play a central role in muscle regeneration, but their quantity and function decline with comorbidity of trauma, aging, and muscle diseases. Although transplantation of MuSCs in traumatically injured muscle in the comorbid context of aging or pathology is a strategy to boost muscle regeneration, an effective cell delivery strategy in these contexts has not been developed. We engineered a synthetic hydrogel-based matrix with optimal mechanical, cell-adhesive, and protease-degradable properties that promotes MuSC survival, proliferation, and differentiation. Furthermore, we establish a biomaterial-mediated cell delivery strategy for treating muscle trauma, where intramuscular injections may not be applicable. Delivery of MuSCs in the engineered matrix significantly improved in vivo cell survival, proliferation, and engraftment in nonirradiated and immunocompetent muscles of aged and dystrophic mice compared to collagen gels and cell-only controls. This platform may be suitable for treating craniofacial and limb muscle trauma, as well as postoperative wounds of elderly and dystrophic patients.
UR - http://www.scopus.com/inward/record.url?scp=85052200928&partnerID=8YFLogxK
U2 - 10.1126/sciadv.aar4008
DO - 10.1126/sciadv.aar4008
M3 - Article
C2 - 30116776
AN - SCOPUS:85052200928
SN - 2375-2548
VL - 4
JO - Science advances
JF - Science advances
IS - 8
M1 - eaar4008
ER -