Synthesis of 3H‐ and 14C‐labeled tracers for studies of functional monoamine oxidase activity

Christer Halldin; Peter Bjurling; Bengt Långström; Robert R. Macgregor; Joanna S. Fowler; Alfred P. Wolf

doi:10.1002/jlcr.2580250508

Synthesis of ³H‐ and ¹⁴C‐labeled tracers for studies of functional monoamine oxidase activity

Christer Halldin, Peter Bjurling, Bengt Långström, Robert R. Macgregor, Joanna S. Fowler, Alfred P. Wolf

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The ³H‐labeling of clorgyline, L‐deprenyl, N,N‐dimethylphenethyl amine and N‐methylphenethyl amine as well as the ¹⁴C‐labeling of clorgyline and L‐deprenyl are described. Labeling was accomplished by N‐alkylation of the free base of the corresponding desmethyl compound, using ³H‐methyl iodide, or by a reductive alkylation using ¹⁴C‐formaldehyde. The crude products were purified by semipreparative HPLC. The total radiochemical yield was 31‐65% based on ³H‐methyl iodide and 60% based on ¹⁴C‐formaldehyde. The radiochemical purity was greater than 99%.

Original language	English
Pages (from-to)	545-552
Number of pages	8
Journal	Journal of Labelled Compounds and Radiopharmaceuticals
Volume	25
Issue number	5
DOIs	https://doi.org/10.1002/jlcr.2580250508
State	Published - May 1988
Externally published	Yes

Keywords

C‐labeling
H‐labeling
L‐deprenyl
N,N‐dimethylphenethyl amine
N‐methylphenethyl amine
clorgyline

Access to Document

10.1002/jlcr.2580250508

Cite this

@article{1ea427dbbdd34d41bcc1476ea56a1aee,

title = "Synthesis of 3H‐ and 14C‐labeled tracers for studies of functional monoamine oxidase activity",

abstract = "The 3H‐labeling of clorgyline, L‐deprenyl, N,N‐dimethylphenethyl amine and N‐methylphenethyl amine as well as the 14C‐labeling of clorgyline and L‐deprenyl are described. Labeling was accomplished by N‐alkylation of the free base of the corresponding desmethyl compound, using 3H‐methyl iodide, or by a reductive alkylation using 14C‐formaldehyde. The crude products were purified by semipreparative HPLC. The total radiochemical yield was 31‐65% based on 3H‐methyl iodide and 60% based on 14C‐formaldehyde. The radiochemical purity was greater than 99%.",

keywords = "C‐labeling, H‐labeling, L‐deprenyl, N,N‐dimethylphenethyl amine, N‐methylphenethyl amine, clorgyline",

author = "Christer Halldin and Peter Bjurling and Bengt L{\aa}ngstr{\"o}m and Macgregor, {Robert R.} and Fowler, {Joanna S.} and Wolf, {Alfred P.}",

year = "1988",

month = may,

doi = "10.1002/jlcr.2580250508",

language = "English",

volume = "25",

pages = "545--552",

journal = "Journal of Labelled Compounds and Radiopharmaceuticals",

issn = "0362-4803",

publisher = "John Wiley and Sons Ltd",

number = "5",

}

TY - JOUR

T1 - Synthesis of 3H‐ and 14C‐labeled tracers for studies of functional monoamine oxidase activity

AU - Halldin, Christer

AU - Bjurling, Peter

AU - Långström, Bengt

AU - Macgregor, Robert R.

AU - Fowler, Joanna S.

AU - Wolf, Alfred P.

PY - 1988/5

Y1 - 1988/5

N2 - The 3H‐labeling of clorgyline, L‐deprenyl, N,N‐dimethylphenethyl amine and N‐methylphenethyl amine as well as the 14C‐labeling of clorgyline and L‐deprenyl are described. Labeling was accomplished by N‐alkylation of the free base of the corresponding desmethyl compound, using 3H‐methyl iodide, or by a reductive alkylation using 14C‐formaldehyde. The crude products were purified by semipreparative HPLC. The total radiochemical yield was 31‐65% based on 3H‐methyl iodide and 60% based on 14C‐formaldehyde. The radiochemical purity was greater than 99%.

AB - The 3H‐labeling of clorgyline, L‐deprenyl, N,N‐dimethylphenethyl amine and N‐methylphenethyl amine as well as the 14C‐labeling of clorgyline and L‐deprenyl are described. Labeling was accomplished by N‐alkylation of the free base of the corresponding desmethyl compound, using 3H‐methyl iodide, or by a reductive alkylation using 14C‐formaldehyde. The crude products were purified by semipreparative HPLC. The total radiochemical yield was 31‐65% based on 3H‐methyl iodide and 60% based on 14C‐formaldehyde. The radiochemical purity was greater than 99%.

KW - C‐labeling

KW - H‐labeling

KW - L‐deprenyl

KW - N,N‐dimethylphenethyl amine

KW - N‐methylphenethyl amine

KW - clorgyline

UR - http://www.scopus.com/inward/record.url?scp=0023904278&partnerID=8YFLogxK

U2 - 10.1002/jlcr.2580250508

DO - 10.1002/jlcr.2580250508

M3 - Article

AN - SCOPUS:0023904278

SN - 0362-4803

VL - 25

SP - 545

EP - 552

JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

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