Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells.

F. A. Laski, R. Belagaje, R. M. Hudziak, M. R. Capecchi, G. P. Norton, P. Palese, U. L. RajBhandary, P. A. Sharp

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


We have used site-specific mutagenesis to change the anticodon of a Xenopus laevis tyrosine tRNA gene so that it would recognize ochre codons. This tRNA gene is expressed when amplified in monkey cells as part of a SV40 recombinant and efficiently suppresses termination at both the ochre codon separating the adenovirus 2 hexon gene from a 23-kd downstream gene and the ochre codon at the end of the NS1 gene of influenza virus A/Tex/1/68. Termination at an amber codon of a NS1 gene of another influenza virus strain was not suppressed by the (Su+) ochre gene suggesting that in mammalian cells amber codons are not recognized by ochre suppressor tRNAs. Finally, microinjection into mammalian cells of both (Su+) ochre tRNA genes and selectible genes containing ochre nonsense mutations gives rise to colonies under selective conditions. We conclude that it should be possible to isolate a wide assortment of mammalian cell lines with ochre suppressor activity.

Original languageEnglish
Pages (from-to)2445-2452
Number of pages8
JournalEMBO Journal
Issue number11
StatePublished - Nov 1984


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