TY - JOUR
T1 - Synthesis and optimize reaction conditions of chlorogenic acid artificial antigen
AU - Lin, Hui
AU - Liu, Ping
AU - Wang, Jianxun
AU - Li, Bin
AU - Mu, Lihua
AU - Hu, Yuan
PY - 2009/8
Y1 - 2009/8
N2 - Objective: To synthesize artificial antigen of the chlorogenic acid (CGA), a constituent in Traditional Chinese Medicine, then optimize the reaction conditions, so that to supply the basis in material and technique for study on Chinese medicine injection of adverse reactions in-depth. Method: The complete antigen of chlorogenic acid (CGA-BSA) was synthesized by CGA coupled to carrier protein bovine serum albumi (BSA) using carbodiimide method. We used the orthogonal design test to optimize reaction conditions and used the MALDI-TOF-MS and UV motheds to determine the coupling rate of the CGA-BSA. Result: The coupling rate were 20 and 18.8, counting by MALDI-TOF-MS and UV, respectively; while the best reaction conditions were NHS: CGA, EDC: CGA, CGA: BSA were 4:1, 1:1, 200:1, respectively, with the pH 9. Conclusion: The complete antigen is preparated successfully. Which supplied the basis to advanced study of correlation.
AB - Objective: To synthesize artificial antigen of the chlorogenic acid (CGA), a constituent in Traditional Chinese Medicine, then optimize the reaction conditions, so that to supply the basis in material and technique for study on Chinese medicine injection of adverse reactions in-depth. Method: The complete antigen of chlorogenic acid (CGA-BSA) was synthesized by CGA coupled to carrier protein bovine serum albumi (BSA) using carbodiimide method. We used the orthogonal design test to optimize reaction conditions and used the MALDI-TOF-MS and UV motheds to determine the coupling rate of the CGA-BSA. Result: The coupling rate were 20 and 18.8, counting by MALDI-TOF-MS and UV, respectively; while the best reaction conditions were NHS: CGA, EDC: CGA, CGA: BSA were 4:1, 1:1, 200:1, respectively, with the pH 9. Conclusion: The complete antigen is preparated successfully. Which supplied the basis to advanced study of correlation.
KW - Chlorogenic acid
KW - Complete antigen
KW - MALDI-TOF-MS
UR - http://www.scopus.com/inward/record.url?scp=70350645629&partnerID=8YFLogxK
M3 - Article
C2 - 19894531
AN - SCOPUS:70350645629
SN - 1001-5302
VL - 34
SP - 1906
EP - 1909
JO - Zhongguo Zhongyao Zazhi
JF - Zhongguo Zhongyao Zazhi
IS - 15
ER -