Synchronization of mouse L-P59 cells by centrifugal elutriation separation

M. L. Meistrich, R. E. Meyn, B. Barlogie

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73 Scopus citations

Abstract

Cell separation by centrifugal elutriation was employed to obtain synchronized cells from asynchronous populations of mouse L-P59 fibroblast cells. The fractions most enriched in specific phases of the cell cycle contained about 90% G1-phase cells, 70% S phase cells and 60% G2 + M phase cells, respectively, as determined by pulse cytophotometry and autoradiography. When replated, cells from separated fractions divided synchronously in culture. The method is rapid and large numbers of cells (3 × 108) can be processed in a single run with no loss of viability. This method should be applicable to most cell lines which can be obtained in single-cell suspension.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalExperimental Cell Research
Volume105
Issue number1
DOIs
StatePublished - 1 Mar 1977
Externally publishedYes

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