@article{8e59f81e12ae4515a8509eab097e8b2e,
title = "Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance",
abstract = "The asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a) acts as a repressive mark that antagonizes trimethylation of H3 lysine 4. Here we report that H3R2 is also symmetrically dimethylated (H3R2me2s) by PRMT5 and PRMT7 and present in euchromatic regions. Profiling of H3-tail interactors by SILAC MS revealed that H3R2me2s excludes binding of RBBP7, a central component of co-repressor complexes Sin3a, NURD and PRC2. Conversely H3R2me2s enhances binding of WDR5, a common component of the coactivator complexes MLL, SET1A, SET1B, NLS1 and ATAC. The interaction of histone H3 with WDR5 distinguishes H3R2me2s from H3R2me2a, which impedes the recruitment of WDR5 to chromatin. The crystallographic structure of WDR5 and the H3R2me2s peptide elucidates the molecular determinants of this high affinity interaction. Our findings identify H3R2me2s as a previously unknown mark that keeps genes poised in euchromatin for transcriptional activation upon cell-cycle withdrawal and differentiation in human cells.",
author = "Valentina Migliori and Julius M{\"u}ller and Sameer Phalke and Diana Low and Marco Bezzi and Mok, {Wei Chuen} and Sahu, {Sanjeeb Kumar} and Jayantha Gunaratne and Paola Capasso and Christian Bassi and Valentina Cecatiello and {De Marco}, Ario and Walter Blackstock and Vladimir Kuznetsov and Bruno Amati and Marina Mapelli and Ernesto Guccione",
note = "Funding Information: We are grateful to C.L. Wei, H. Thoreau and Z.H. Yap for help with the Solexa high-throughput sequencing; to P. Rorth and X. Yang for the use of the Drosophila facility; to S. Choksi (Institute of Molecular and Cell Biology (IMCB)) for providing the zebrafish total protein extract; to V. Do Dang and X.Y. Fu (National University of Singapore) for sharing the PSuper vector targeting WDR77 and to N. Jennifer for technical help. We thank M. Walsh, P. Kaldis, X.Y. Fu and S. Choksi for discussions and P. Rorth, G. Gargiulo, M.M. Zhou and M. Walsh for critically reading the manuscript. This work was supported by the IMCB, A-STAR core funding to E.G.",
year = "2012",
month = feb,
doi = "10.1038/nsmb.2209",
language = "English",
volume = "19",
pages = "136--145",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "2",
}