SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts

Yu Tzu Chang, Chung Li Shu, Jing Yang Lai, Ching Yu Lin, Chih Pin Chuu, Kazuhiro Morishita, Tomonaga Ichikawa, Rolf Jessberger, Yasuhisa Fukui

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Mouse embryo fibroblasts (MEFs) grow slowly after cultivation from animals, however, after an extended period of cultivation, their growth accelerates. We found that SWAP-70 deficient MEFs failed to increase growth rates. They maintain normal growth rates and proliferation cycles for at least 5 years. Complementing SWAP-70 deficiency in one of these MEF clones, MEF1F2, by expressing human SWAP-70 resulted in fast growth of the cells after further cultivation for a long period. The resulting cells show a transformation phenotype, since they grow on top of each other and do not show contact inhibition. This phenotype was reverted when sanguinarine, a putative SWAP-70 inhibitor, was added. Two SWAP-70 expressing clones were examined in detail. Even after cell density became very high their cdc2 and NFκB were still activated suggesting that they do not stop growing. One of the clones formed colonies in soft agar and formed tumors in nude mice. Lately, one more clone became transformed being able to make colonies in soft agar. We maintain 4 human SWAP-70 expressing MEF1F2 cell lines. Three out of 4 clones exhibited transforming phenotypes. The mouse SWAP-70 gene also promoted transformation of MEFs. Taken together our data suggest that SWAP-70 is not a typical oncogene, but is required for spontaneous transformation of MEFs.

Original languageEnglish
Pages (from-to)150-157
Number of pages8
JournalExperimental Cell Research
Volume345
Issue number2
DOIs
StatePublished - 15 Jul 2016
Externally publishedYes

Keywords

  • Mouse embryo fibroblasts
  • SWAP-70
  • Soft agar colony formation
  • Transformation
  • Tumor formation

Fingerprint

Dive into the research topics of 'SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts'. Together they form a unique fingerprint.

Cite this