Abstract
We describe a general method for incorporating target ligands into the surface of biocompatible polyester poly(lactic-co-glycolic acid) (PLGA) 50/50 materials using fatty acids. Avidin-fatty acid conjugates were prepared and efficiently incorporated into PLGA. Avidin was chosen as an adaptor protein to facilitate the attachment of a variety of biotinylated ligands. We show that fatty acid preferentially associates with the hydrophobic PLGA matrix, rather than the external aqueous environment, facilitating a prolonged presentation of avidin over several weeks. We successfully applied this approach in both microspheres encapsulating a model protein, bovine serum albumin, and PLGA scaffolds fabricated by a salt-leaching method. Because of its ease, generality and flexibility, this strategy promises widespread utility in modifying the surface of PLGA-based materials for applications in drug delivery and tissue engineering.
Original language | English |
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Pages (from-to) | 5727-5736 |
Number of pages | 10 |
Journal | Biomaterials |
Volume | 26 |
Issue number | 28 |
DOIs | |
State | Published - Oct 2005 |
Externally published | Yes |
Keywords
- Biocompatibility
- Biodegradation
- Drug delivery
- Lipid
- Microsphere
- Scaffold
- Surface modification