TY - JOUR
T1 - Surface-aerosol stability and pathogenicity of diverse middle east respiratory syndrome coronavirus strains, 2012-2018
AU - van Doremalen, Neeltje
AU - Letko, Michael
AU - Fischer, Robert J.
AU - Bushmaker, Trenton
AU - Schulz, Jonathan
AU - Yinda, Claude K.
AU - Seifert, Stephanie N.
AU - Kim, Nam Joong
AU - Hemida, Maged G.
AU - Kayali, Ghazi
AU - Park, Wan Beom
AU - Perera, Ranawaka A.P.M.
AU - Tamin, Azaibi
AU - Thornburg, Natalie J.
AU - Tong, Suxiang
AU - Queen, Krista
AU - van Kerkhove, Maria D.
AU - Ki Choi, Young
AU - Oh, Myoung Don
AU - Assiri, Abdullah M.
AU - Peiris, Malik
AU - Gerber, Susan I.
AU - Munster, Vincent J.
N1 - Publisher Copyright:
© 2021 Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2021/12
Y1 - 2021/12
N2 - Middle East respiratory syndrome coronavirus (MERS-CoV) infects humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. Although some mutations found in camel-derived MERS-CoV strains have been characterized, most natural variation found across MERS-CoV isolates remains unstudied. We report on the environmental stability, replication kinetics, and pathogenicity of several diverse isolates of MERS-CoV, as well as isolates of severe acute respiratory syndrome coronavirus 2, to serve as a basis of comparison with other stability studies. Although most MERS-CoV isolates had similar stability and pathogenicity in our experiments, the camel-derived isolate C/KSA/13 had reduced surface stability, and another camel isolate, C/BF/15, had reduced pathogenicity in a small animal model. These results suggest that although betacoronaviruses might have similar environmental stability profiles, individual variation can influence this phenotype, underscoring the need for continual global viral surveillance.
AB - Middle East respiratory syndrome coronavirus (MERS-CoV) infects humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. Although some mutations found in camel-derived MERS-CoV strains have been characterized, most natural variation found across MERS-CoV isolates remains unstudied. We report on the environmental stability, replication kinetics, and pathogenicity of several diverse isolates of MERS-CoV, as well as isolates of severe acute respiratory syndrome coronavirus 2, to serve as a basis of comparison with other stability studies. Although most MERS-CoV isolates had similar stability and pathogenicity in our experiments, the camel-derived isolate C/KSA/13 had reduced surface stability, and another camel isolate, C/BF/15, had reduced pathogenicity in a small animal model. These results suggest that although betacoronaviruses might have similar environmental stability profiles, individual variation can influence this phenotype, underscoring the need for continual global viral surveillance.
UR - https://www.scopus.com/pages/publications/85120308869
U2 - 10.3201/eid2712.210344
DO - 10.3201/eid2712.210344
M3 - Article
C2 - 34808078
AN - SCOPUS:85120308869
SN - 1080-6040
VL - 27
SP - 3052
EP - 3062
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 12
ER -