Suppression of type IV collagenase in MDA-MB-435 human breast cancer cells by eicosapentaenoic acid in vitro and in vivo

Xin Hua Liu, David P. Rose

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Dietary supplementation with 4% eicosapentaenoic acid (EPA), a long-chain polyunsaturated fatty acid, suppressed the development of lung metastases in nude mice from MDA-MB-435 human breast cancer cell mammary fat pad solid tumors. Zymography of primary tumor homogenates showed that this inhibition of metastasis was associated with reduced levels of 92-kDa type IV collagenase gelatinolytic activity; this isoform has been previously associated with the metastatic phenotype. The 72-kDa isoform, which was also present, may have arisen from the cancer cells, or tumor-associated host cells. In vitro, the incorporation of EPA, 0.25-1.0 μg/ml into the medium caused a concentration-related suppression of cultured MDA-MB-435 cell 92-kDa type IV collagenase mRNA expression.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalCancer Letters
Volume92
Issue number1
DOIs
StatePublished - 25 May 1995
Externally publishedYes

Keywords

  • Breast cancer
  • Collagenase
  • Eicosapentaenoic acid
  • Metastasis

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