Supported aqueous-phase enzymatic catalysis in organic media

Sanghamitra Parida, Ravindra Datta, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The use of partially hydrated porous silica particles has been studied as a support for cofactor-dependent enzymatic catalysis in organic solvents. At an optimal pore hydration corresponding to 70% pore volume, horse liver alcohol dehydrogenase catalyzes the oxidation and reduction of alcohols and aldehydes, respectively, with rates sixfold higher than with nonporous glass beads as the enzymatic support and with cofactor recycling numbers in excess of 105. Thus, supported aqueous-phase enzymatic catalysis makes highly effective use of the enzyme and cofactor by coimmobilization and by providing a high interfacial area for reactions in organic media.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalApplied Biochemistry and Biotechnology
Volume33
Issue number1
DOIs
StatePublished - Apr 1992
Externally publishedYes

Keywords

  • Supported liquid-phase catalysis
  • alcohol dehydrogenase
  • organic media

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