TY - JOUR
T1 - Superinfection of EBV-carrying lines with Herpes virus papio (HVP)
T2 - Induction of early antigen (EA) and inhibition of cellular DNA synthesis
AU - Steinitz, Michael
AU - Klein, George
N1 - Funding Information:
Two LABORATORY substrains of the Epstein-Barr virus (EBV), derived from the B95-8 \[1\] and the P3HR-1 lines \[2\] have been studied most extensively. They show considerable difference in their biological properties. B95-8 virus induces the nuclear antigen EBNA and stimulates DNA synthesis in normal B lymphocytes of human or some non-human primate origin. This is tbllowed by transformation (immortalization) into permanent lines \[1, 3\]. In contrast, P3HR-1 does not transform, and fails to induce EBNA and cellular DNA synthesis \[3\]. There is less of a difference in the action of the two viruses on the EBV negative but EBV-susceptible BJAB and Ramos lines \[4, 5\]. They could be converted into permanent EBV-carriers by both virus substrains \[6, 7\]. Nevertheless, there are considerable differences between the two virus strains even on established B-cell lines. EBV-receptor carrying lines are inhibited by P3HR-1 but not by B95-8 virus \[8\]. The P3HR-1 virus appears to inhibit the cellular DNA synthesis in them. A similar inhibitory action on cellular DNA synthesis was found *This project has been funded in part with Federal funds from the Department of Health, Education and Welfare under contract number NOI CP 33316 and by the Swedish Cancer Society.
PY - 1979/2
Y1 - 1979/2
N2 - An Epstein-Barr virus (EBV) related herpes virus H. papio (HVP) derived from 9B cell line was shown to inhibit the DNA synthesis in B-cell mitogen stimulated human lymphocytes, in B95-8 virus exposed human lymphocytes, and the DNA metabolism of EBV-receptor positive cell lines. The inhibition could be abolished by a Burkitt serum known to have a high anti-EBV titer. Similar inhibition activities were shown for P3HR-1 virus. The relationship between the inability to immortalize normal cells, the ability to induce early antigen (EA) in certain cell lines and the DNA inhibitory effect of both 9B and P3HR-1 viruses is discussed.
AB - An Epstein-Barr virus (EBV) related herpes virus H. papio (HVP) derived from 9B cell line was shown to inhibit the DNA synthesis in B-cell mitogen stimulated human lymphocytes, in B95-8 virus exposed human lymphocytes, and the DNA metabolism of EBV-receptor positive cell lines. The inhibition could be abolished by a Burkitt serum known to have a high anti-EBV titer. Similar inhibition activities were shown for P3HR-1 virus. The relationship between the inability to immortalize normal cells, the ability to induce early antigen (EA) in certain cell lines and the DNA inhibitory effect of both 9B and P3HR-1 viruses is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0018349028&partnerID=8YFLogxK
U2 - 10.1016/0014-2964(79)90063-X
DO - 10.1016/0014-2964(79)90063-X
M3 - Article
C2 - 220052
AN - SCOPUS:0018349028
SN - 0014-2964
VL - 15
SP - 217
EP - 222
JO - European Journal of Cancer (1965)
JF - European Journal of Cancer (1965)
IS - 2
ER -