Abstract
A fluorescent derivative of GM1-ganglioside was synthesized by linking sulforhodamine 101 to the sphingosine moiety through amino dodecanoyl residue. The product (SR-12GM1) was quantitatively converted to SR-12GM2 by treatment with bovine testes β-galactosidase and in intact cultured human skin fibroblasts was catabolized to sulforhodamine GM2, GM3 and ceramide; the latter product was further converted to sphingomyelin. In aqueous medium SR-12GM1 formed micelles. When transfer from micelles to vesicles and between vesicles was compared with that of pyrene-GM1, the transfer of SR-12GM1 occurred at higher rates, following in both cases a biexponential curve.
Original language | English |
---|---|
Pages (from-to) | 143-152 |
Number of pages | 10 |
Journal | Chemistry and Physics of Lipids |
Volume | 72 |
Issue number | 2 |
DOIs | |
State | Published - 8 Aug 1994 |
Externally published | Yes |
Keywords
- Fluorescent gangliosides
- GM-ganglioside
- Gangliosides transfer
- Sulforhodamine 101