SU‐GG‐I‐180: Application of Molecular Imaging in the Monitoring of Angiogenesis of a Hindlimb Ischemia Model

G. Kagadis, K. Tsioupinaki, G. Loudos, A. Diamantopoulos, G. Soultanidis, D. Karnabatidis, D. Mihailidis, G. Nikiforidis

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: Avβ3 integrin expression is a characteristic feature of the angiogenic phenotype of endothelial cells. Targeted molecular imaging of integrins may be a further step forward in the evaluation of specific and early aspects of angiogenesis. The purpose of our study was to investigate the potential of molecular imaging with the aid of a dedicated high resolution γ camera in the rabbit hindlimb ischemia model. Method and Materials: A 99mTc labeled cyclic RGD peptide ([c RGDfk‐His]‐99mTc) was employed for angiogenesis imaging in the New Zealand white rabbits hindlimb ischemia model. Imaging of αvβ3 expression was performed in two animal groups. In group A imaging was employed 3 days and in group B 9 days post femoral artery occlusion (2 rabbits in each group). Each rabbit was injected intravenously with 200 μl of [c RGDfk‐His]‐99mTc (0.5 mCi). Consequently dynamic planar imaging was performed with a high resolution gamma camera with FOV 5×10 cm, equipped with a parallel hole collimator. Static 8 minute images at 80 minutes post injection of the radiotracer were acquired. Results: We have imaged 4 rabbits. An increase of 12.2% was observed in group A in relative radiotracer retention at the ischemic hindlimb compared to control. In group B this relative radiotracer retention was increased to 25.8%. The control limbs showed an increase in the radiotracer retention at day 9 but still much lower compared to ischemic limbs. Moreover, radiotracer accumulation was observed to a region distal to the original area of occlusion, where the newly formed proximal collaterals are located. Conclusion: Our study demonstrates that the phenomenon of angiogenesis proceeds and is maximized within on day 9 following induction of ischemia in a mammalian experimental model. Further studies are deemed necessary in order to establish this conclusion and provide quantitative results.

Original languageEnglish
Pages (from-to)3143
Number of pages1
JournalMedical Physics
Volume37
Issue number6
DOIs
StatePublished - Jun 2010
Externally publishedYes

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