Sudden Cardiac Death Due to Deficiency of the Mitochondrial Inorganic Pyrophosphatase PPA2

Hannah Kennedy, Tobias B B. Haack, Verity Hartill, Lavinija Mataković, E. Regula Baumgartner, Howard Potter, Richard Mackay, Charlotte L L. Alston, Siobhan O'Sullivan, Robert McFarland, Grainne Connolly, Caroline Gannon, Richard King, Scott Mead, Ian Crozier, Wandy Chan, Chris M M. Florkowski, Martin Sage, Thomas Höfken, Bader AlhaddadLaura S S. Kremer, Robert Kopajtich, René G G. Feichtinger, Wolfgang Sperl, Richard J J. Rodenburg, Jean Claude C. Minet, Angus Dobbie, Tim M M. Strom, Thomas Meitinger, Peter M M. George, Colin A A. Johnson, Robert W W. Taylor, Holger Prokisch, Kit Doudney, Johannes A A. Mayr

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We have used whole-exome sequencing in ten individuals from four unrelated pedigrees to identify biallelic missense mutations in the nuclear-encoded mitochondrial inorganic pyrophosphatase (PPA2) that are associated with mitochondrial disease. These individuals show a range of severity, indicating that PPA2 mutations may cause a spectrum of mitochondrial disease phenotypes. Severe symptoms include seizures, lactic acidosis, cardiac arrhythmia, and death within days of birth. In the index family, presentation was milder and manifested as cardiac fibrosis and an exquisite sensitivity to alcohol, leading to sudden arrhythmic cardiac death in the second decade of life. Comparison of normal and mutant PPA2-containing mitochondria from fibroblasts showed that the activity of inorganic pyrophosphatase was significantly reduced in affected individuals. Recombinant PPA2 enzymes modeling hypomorphic missense mutations had decreased activity that correlated with disease severity. These findings confirm the pathogenicity of PPA2 mutations and suggest that PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function than previously recognized.

Original languageEnglish
Pages (from-to)674-682
Number of pages9
JournalAmerican Journal of Human Genetics
Volume99
Issue number3
DOIs
StatePublished - 1 Sep 2016
Externally publishedYes

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