Objective: To describe two successive pregnancies resulting in two healthy infants in an HIV-discordant couple who underwent IVF-ICSI. Design: Case report. Setting: University-based infertility clinic. Patient(s): A couple seeking fertility treatment in which the male partner was seropositive for HIV-1. Intervention(s): Controlled ovarian hyperstimulation and IVF-ET with ICSI. Main Outcome Measure(s): Pregnancy outcome and HIV-1 seroconversion. Result(s): The couple delivered two healthy infants on successive pregnancies with use of IVF-ET with ICSI. Conclusion(s): For HIV-discordant couples in which the male partner is seropositive for HIV-1, IVF with ICSI is a viable option.