Successful production of intrathyroidal human t cell hybridomas: Evidence for intact helper t cell function in graves’ disease

A. Martin, A. E. Schwartz, E. W. Friedman, T. F. Davies

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have developed a series of human intrathyroidal T-T cell hybridomas and evaluated their phenotypic characteristics and lymphokine secretions in order to further understand the role of the T cell in Graves’ disease. Mitogen-stimulated T cell blasts were generated from intrathyroidal lymphocyte preparations and fused with a hypoxanthine-, aminopterin-, and thymidine-sensitive variant of the Molt 4 human leukemia T cell line. The resulting intrathyroidal T-T cell hybridomas and T-T cell hybridomas obtained from normal peripheral blood mitogen-stimulated T cell blasts were expanded and tested for their biological function. None of the generated T cell hybridomas exhibited antigen-specific IL-2 secretion when stimulated with autologous thyrocytes, although 60% of thehybridomas expressed CD3 antigen and the T cell receptor α/β heterodimer. However, 9 intrathyroidal and 11 peripheral blood T cell hybridomas secreted a factor(s) that significantly enhanced immunoglobulin G secretion in vitro (P < 0.005, by Student-Newman-Keuls test; mean ± SEM, 338 ± 60% increase). In summary, we have successfully used a technique that allows the construction of T-T cell hybridomas derived from intrathyroidal T cell cultures. The data demonstrated that a predominance of helper factor-secreting T cells were available for fusion within the Graves’ thyroid gland. Such observations are further evidence for intact T cell help within the thyroid gland patients with Graves’ disease.

Original languageEnglish
Pages (from-to)1104-1108
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume69
Issue number6
DOIs
StatePublished - Dec 1989

Fingerprint

Dive into the research topics of 'Successful production of intrathyroidal human t cell hybridomas: Evidence for intact helper t cell function in graves’ disease'. Together they form a unique fingerprint.

Cite this