Subunit-specific inhibition of acid sensing ion channels by stomatin-like protein 1

Alexey Kozlenkov, Liudmila Lapatsina, Gary R. Lewin, Ewan St John Smith

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

There are five mammalian stomatin-domain genes, all of which encode peripheral membrane proteins that can modulate ion channel function. Here we examined the ability of stomatin-like protein 1 (STOML1) to modulate the proton-sensitive members of the acid-sensing ion channel (ASIC) family. STOML1 profoundly inhibits ASIC1a, but has no effect on the splice variant ASIC1b. The inactivation time constant of ASIC3 is also accelerated by STOML1. We examined STOML1 null mutant mice with a β-galactosidase-neomycin cassette gene-trap reporter driven from the STOML1 gene locus, which indicated that STOML1 is expressed in at least 50% of dorsal root ganglion (DRG) neurones. Patch clamp recordings from mouse DRG neurones identified a trend for larger proton-gated currents in neurones lacking STOML1, which was due to a contribution of effects upon both transient and sustained currents, at different pH, a finding consistent with an endogenous inhibitory function for STOML1.

Original languageEnglish
Pages (from-to)557-569
Number of pages13
JournalJournal of Physiology
Volume592
Issue number4
DOIs
StatePublished - 15 Feb 2014

Fingerprint

Dive into the research topics of 'Subunit-specific inhibition of acid sensing ion channels by stomatin-like protein 1'. Together they form a unique fingerprint.

Cite this