Subsurface tumor progression investigated by noninvasive optical second harmonic tomography

Yici Guo; Howard E. Savage; Feng Liu; Stimson P. Schantz; P. P. Ho; R. R. Alfano

doi:10.1073/pnas.96.19.10854

Subsurface tumor progression investigated by noninvasive optical second harmonic tomography

Yici Guo, Howard E. Savage, Feng Liu, Stimson P. Schantz, P. P. Ho, R. R. Alfano

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Nonlinear optical imaging with femtosecond (10^-15-second) laser technology was used to evaluate the subsurface tumor progression in control, dysplasia, and cancerous 7,12-dimethylbenz[a]anthracene-treated hamster cheek pouch mucosa tissues. Two-dimensional images of hamster cheek pouch mucosa tissues were obtained by scanning the second harmonic signal at various sagittal and axial positions. The spatial mapping of the second harmonic signals showed depth differentiation between normal, dysplasia, and a more advanced cancerous state. This nonlinear optical method offers a noninvasive in situ imaging tool to the medical community.

Original language	English
Pages (from-to)	10854-10856
Number of pages	3
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	96
Issue number	19
DOIs	https://doi.org/10.1073/pnas.96.19.10854
State	Published - 14 Sep 1999
Externally published	Yes

Keywords

Histology
Optical tomography
Second harmonic generation

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10.1073/pnas.96.19.10854

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title = "Subsurface tumor progression investigated by noninvasive optical second harmonic tomography",

abstract = "Nonlinear optical imaging with femtosecond (10-15-second) laser technology was used to evaluate the subsurface tumor progression in control, dysplasia, and cancerous 7,12-dimethylbenz[a]anthracene-treated hamster cheek pouch mucosa tissues. Two-dimensional images of hamster cheek pouch mucosa tissues were obtained by scanning the second harmonic signal at various sagittal and axial positions. The spatial mapping of the second harmonic signals showed depth differentiation between normal, dysplasia, and a more advanced cancerous state. This nonlinear optical method offers a noninvasive in situ imaging tool to the medical community.",

keywords = "Histology, Optical tomography, Second harmonic generation",

author = "Yici Guo and Savage, {Howard E.} and Feng Liu and Schantz, {Stimson P.} and Ho, {P. P.} and Alfano, {R. R.}",

year = "1999",

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doi = "10.1073/pnas.96.19.10854",

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T1 - Subsurface tumor progression investigated by noninvasive optical second harmonic tomography

AU - Guo, Yici

AU - Savage, Howard E.

AU - Liu, Feng

AU - Schantz, Stimson P.

AU - Ho, P. P.

AU - Alfano, R. R.

PY - 1999/9/14

Y1 - 1999/9/14

N2 - Nonlinear optical imaging with femtosecond (10-15-second) laser technology was used to evaluate the subsurface tumor progression in control, dysplasia, and cancerous 7,12-dimethylbenz[a]anthracene-treated hamster cheek pouch mucosa tissues. Two-dimensional images of hamster cheek pouch mucosa tissues were obtained by scanning the second harmonic signal at various sagittal and axial positions. The spatial mapping of the second harmonic signals showed depth differentiation between normal, dysplasia, and a more advanced cancerous state. This nonlinear optical method offers a noninvasive in situ imaging tool to the medical community.

AB - Nonlinear optical imaging with femtosecond (10-15-second) laser technology was used to evaluate the subsurface tumor progression in control, dysplasia, and cancerous 7,12-dimethylbenz[a]anthracene-treated hamster cheek pouch mucosa tissues. Two-dimensional images of hamster cheek pouch mucosa tissues were obtained by scanning the second harmonic signal at various sagittal and axial positions. The spatial mapping of the second harmonic signals showed depth differentiation between normal, dysplasia, and a more advanced cancerous state. This nonlinear optical method offers a noninvasive in situ imaging tool to the medical community.

KW - Histology

KW - Optical tomography

KW - Second harmonic generation

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Subsurface tumor progression investigated by noninvasive optical second harmonic tomography

Abstract

Keywords

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