Abstract
Rat Brain has a lipase which hydrolyzes diacylglycerol at an optimal pH of 4.8 (1). The subcellular distribution of this acid diacylglycerol lipase was studied in brain tissue of rats and mice; in the latter case neurological mutants and their normal controls were used. Several other acidic hydrolases were employed as lysosomal markers. In mouse brain, the specific activity which is about 50-100 times lower than in rat brain, was greatest in the lysosomal fraction. In contrast, no enrichment of DG-lipase was observed in any subcellular fraction of the active enzyme of rat brain. Activities were about equally distributed in the microsomal, myelin-synaptosomal and lysosomal fractions.
| Original language | English |
|---|---|
| Pages (from-to) | 417-422 |
| Number of pages | 6 |
| Journal | Neurochemical Research |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1983 |
| Externally published | Yes |