@inbook{9fc9439a94d24957ad2d12be3799f921,
title = "Studying evolutionary adaptation of MERS-CoV",
abstract = "Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018.",
keywords = "Cell culture, Experimental evolution, Forced adaptation, Host restriction, MERS-CoV, Semi-permissive cell line, Species barrier",
author = "Michael Letko and Vincent Munster",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0211-9\_1",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "3--8",
booktitle = "Methods in Molecular Biology",
}