Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay

Xiangli Liu; Yougang Mao; Errol V. Mathias; Collin Ma; Osmundo Franco; Yong Ba; Julia A. Kornfield; Tieli Wang; Lijun Xue; Bing Sen Zhou; Yun Yen

doi:10.1007/s10971-007-1659-y

Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay

Xiangli Liu, Yougang Mao, Errol V. Mathias, Collin Ma, Osmundo Franco, Yong Ba, Julia A. Kornfield, Tieli Wang, Lijun Xue, Bing Sen Zhou, Yun Yen

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R _f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil-Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic R_f-cores/IPDU shells of the R_f-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the R_f-PEG. It has been known that R_f-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the R_f-PEG's biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R _f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.

Original language	English
Pages (from-to)	269-278
Number of pages	10
Journal	Journal of Sol-Gel Science and Technology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1007/s10971-007-1659-y
State	Published - Mar 2008
Externally published	Yes

Keywords

Chlorambucil
Controlled release
Electron paramagnetic resonance
Polymeric drug depot
Proliferation assay
Rf-PEG hydrogel
Tempol

Access to Document

10.1007/s10971-007-1659-y

Cite this

Liu, X., Mao, Y., Mathias, E. V., Ma, C., Franco, O., Ba, Y., Kornfield, J. A., Wang, T., Xue, L., Zhou, B. S., & Yen, Y. (2008). Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay. Journal of Sol-Gel Science and Technology, 45(3), 269-278. https://doi.org/10.1007/s10971-007-1659-y

@article{6d3ead4d9fb147bd9f489efa270ffa74,

title = "Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay",

abstract = "Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil-Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic Rf-cores/IPDU shells of the Rf-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the Rf-PEG. It has been known that Rf-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the Rf-PEG's biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.",

keywords = "Chlorambucil, Controlled release, Electron paramagnetic resonance, Polymeric drug depot, Proliferation assay, Rf-PEG hydrogel, Tempol",

author = "Xiangli Liu and Yougang Mao and Mathias, {Errol V.} and Collin Ma and Osmundo Franco and Yong Ba and Kornfield, {Julia A.} and Tieli Wang and Lijun Xue and Zhou, {Bing Sen} and Yun Yen",

note = "Funding Information: Acknowledgment This project is supported by the NSF Grant 0351848. Thanks to DOD for funding the EPR spectrometer under the grant d20030904hsi.",

year = "2008",

month = mar,

doi = "10.1007/s10971-007-1659-y",

language = "English",

volume = "45",

pages = "269--278",

journal = "Journal of Sol-Gel Science and Technology",

issn = "0928-0707",

publisher = "Springer Netherlands",

number = "3",

}

Liu, X, Mao, Y, Mathias, EV, Ma, C, Franco, O, Ba, Y, Kornfield, JA, Wang, T, Xue, L, Zhou, BS & Yen, Y 2008, 'Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay', Journal of Sol-Gel Science and Technology, vol. 45, no. 3, pp. 269-278. https://doi.org/10.1007/s10971-007-1659-y

Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay. / Liu, Xiangli; Mao, Yougang; Mathias, Errol V. et al.
In: Journal of Sol-Gel Science and Technology, Vol. 45, No. 3, 03.2008, p. 269-278.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay

AU - Liu, Xiangli

AU - Mao, Yougang

AU - Mathias, Errol V.

AU - Ma, Collin

AU - Franco, Osmundo

AU - Ba, Yong

AU - Kornfield, Julia A.

AU - Wang, Tieli

AU - Xue, Lijun

AU - Zhou, Bing Sen

AU - Yen, Yun

N1 - Funding Information: Acknowledgment This project is supported by the NSF Grant 0351848. Thanks to DOD for funding the EPR spectrometer under the grant d20030904hsi.

PY - 2008/3

Y1 - 2008/3

N2 - Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil-Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic Rf-cores/IPDU shells of the Rf-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the Rf-PEG. It has been known that Rf-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the Rf-PEG's biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.

AB - Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil-Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic Rf-cores/IPDU shells of the Rf-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the Rf-PEG. It has been known that Rf-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the Rf-PEG's biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.

KW - Chlorambucil

KW - Controlled release

KW - Electron paramagnetic resonance

KW - Polymeric drug depot

KW - Proliferation assay

KW - Rf-PEG hydrogel

KW - Tempol

UR - http://www.scopus.com/inward/record.url?scp=39149102826&partnerID=8YFLogxK

U2 - 10.1007/s10971-007-1659-y

DO - 10.1007/s10971-007-1659-y

M3 - Article

AN - SCOPUS:39149102826

SN - 0928-0707

VL - 45

SP - 269

EP - 278

JO - Journal of Sol-Gel Science and Technology

JF - Journal of Sol-Gel Science and Technology

IS - 3

ER -

Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay

Abstract

Keywords

Access to Document

Fingerprint

Cite this