Study on synergistic antidepressant effect of 3,6-disinapoyl sucrose combined with tenuifoliside A and its possible mechanism

Aim: To study the synergistic anti-depression effect of 3, 6-disinapoyl sucrose (DISS) and tenuifoliside A (TFSA) from Radix Polygalae and the preliminary mechanism. Methods: Using the classical behavioral despair and depression model of mouse tail suspension test, 120 mice were divided into control group, positive group, DISS 5 mg · kg^-1 group, DISS 10 mg · kg^-1 group,TFSA 5 mg · kg^-1 group,TFSA 10 mg · kg^-1 group, DISS 5 mg · kg^-1 + TFSA 5 mg · kg^-1 group,DISS 5 mg · kg^-1 + TFSA 10 mg · kg^-1 group,DISS 10 mg · kg^-1 +TFSA 5 mg · kg^-1 group and DISS 10 mg · kg^-1 + TFSA 10 mg · kg^-1 group randomly. They were given intragastric injection for 7 days continuously, to observe the effect of DISS and TFSA monomer and its combination on the time of mouse tail suspension. Expression of BDNF in the hippocampus of mice was detected by immunohistochemis-try. The expressions of CREB, pCREB, CRTC1 and BDNF in the hippocampus of mice were detected by Western blot method. Results The administration of DISS and TFSA could shorten the immobility time of i mice subjected to the tail. DISS(10 mg · kg^-1) and TFSA(10 mg · kg^-1) group were significantly lower than single dose drug group (P <0. 05). DISS and TFSA and the combination groups could increase the expression of BDNF in hippocampus and cortex by immu-nohistochemistry (P < 0. 05). At the same time, the contents of CREB, CRTC1, pCREB and BDNF protein in the hippocampus were increased by DISS and TFSA, and the combination group was significantly higher than the single drug group (P < 0. 05). Conclusion: The administration of DISS and TFSA are used to activate CREB transcription factor CRTC1, and activate the phosphorylation of CREB in the hippocampus, and then increase the expression of BDNF in the hippocampus and plays a synergistic antidepressant effect.