Study on sensitization and mechanism of CGA-BSA

Xiaowu Huang, Hongbo Liao, Ping Liu, Hui Lin, Liyan Yuan, Yuan Hu

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Objective: To investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA). Method: Using intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma. Result: There established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 ± 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA. Conclusion: Allergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.

Original languageEnglish
Pages (from-to)1181-1184
Number of pages4
JournalZhongguo Zhongyao Zazhi
Issue number9
StatePublished - May 2010
Externally publishedYes


  • Allergic response
  • Type I allergy


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