Abstract
AIM: To study the function of newly cloned NF-kappaB-activating gene (NAP2) and its mechanism. METHEDS: Activation of NF-kappaB and IL-12 promoters as well as modulation of IL-12 synthesis by NAP2 in macrophages were observed by NF-kappaB and IL-12 luciferase reporter assay and intracellular IL-12 staining.
RESULTS: NAP2 could activate NF-kappaB and IL-12 promoter. But IkappaBalphaDN could inhibit their activation. In addition, NAP2 could induce IL-12 expression in macrophage cell line J774.
CONCLUSION: NAP2 as a newly cloned NF-kappaB activating gene can activate IL-12 promoter and induce IL-12 expression in macrophages. The data suggest that NAP2 regulates the expression of IL-12 most likely through its modulation of NF-kappaB transcription factor.
Original language | English |
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Pages (from-to) | 403-405 |
Number of pages | 3 |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology |
Volume | 19 |
Issue number | 4 |
State | Published - 1 Jul 2003 |
Externally published | Yes |