Study of immunogenicity of chlorogenic acid-BSA with different number of CGA

Liyan Yuan; Ping Liu; Yuan Hu; Hongbo Liao; Lihua Mu; Hui Lin; Honglin Huang

doi:10.4268/cjcmm20102026

Study of immunogenicity of chlorogenic acid-BSA with different number of CGA

Liyan Yuan, Ping Liu, Yuan Hu, Hongbo Liao, Lihua Mu, Hui Lin, Honglin Huang

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To investigate the immunogenicity of chlorogenic acid-BSA( CGA-BSA) and the foundation for the allergization of CGA-BSA. Method: The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by EUSA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer. Result: When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest. Conclusion: It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.

Original language	English
Pages (from-to)	2756-2759
Number of pages	4
Journal	Zhongguo Zhongyao Zazhi
Volume	35
Issue number	20
DOIs	https://doi.org/10.4268/cjcmm20102026
State	Published - 15 Oct 2010
Externally published	Yes

Keywords

Chlorogenic acid
Holoantigen
Immunogenicity
PCA

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10.4268/cjcmm20102026

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title = "Study of immunogenicity of chlorogenic acid-BSA with different number of CGA",

abstract = "Objective: To investigate the immunogenicity of chlorogenic acid-BSA( CGA-BSA) and the foundation for the allergization of CGA-BSA. Method: The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by EUSA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer. Result: When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest. Conclusion: It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.",

keywords = "Chlorogenic acid, Holoantigen, Immunogenicity, PCA",

author = "Liyan Yuan and Ping Liu and Yuan Hu and Hongbo Liao and Lihua Mu and Hui Lin and Honglin Huang",

year = "2010",

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day = "15",

doi = "10.4268/cjcmm20102026",

language = "English",

volume = "35",

pages = "2756--2759",

journal = "Zhongguo Zhongyao Zazhi",

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TY - JOUR

T1 - Study of immunogenicity of chlorogenic acid-BSA with different number of CGA

AU - Yuan, Liyan

AU - Liu, Ping

AU - Hu, Yuan

AU - Liao, Hongbo

AU - Mu, Lihua

AU - Lin, Hui

AU - Huang, Honglin

PY - 2010/10/15

Y1 - 2010/10/15

N2 - Objective: To investigate the immunogenicity of chlorogenic acid-BSA( CGA-BSA) and the foundation for the allergization of CGA-BSA. Method: The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by EUSA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer. Result: When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest. Conclusion: It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.

AB - Objective: To investigate the immunogenicity of chlorogenic acid-BSA( CGA-BSA) and the foundation for the allergization of CGA-BSA. Method: The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by EUSA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer. Result: When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest. Conclusion: It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.

KW - Chlorogenic acid

KW - Holoantigen

KW - Immunogenicity

KW - PCA

UR - https://www.scopus.com/pages/publications/78751697842

U2 - 10.4268/cjcmm20102026

DO - 10.4268/cjcmm20102026

M3 - Article

C2 - 21246835

AN - SCOPUS:78751697842

SN - 1001-5302

VL - 35

SP - 2756

EP - 2759

JO - Zhongguo Zhongyao Zazhi

JF - Zhongguo Zhongyao Zazhi

IS - 20

ER -

Study of immunogenicity of chlorogenic acid-BSA with different number of CGA

Abstract

Keywords

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