Study of 20 S proteasome activity regulation by histone H3 using multi-mass spectrometric approach

Helena Costa; Andrew Krutchinsky; Brian T. Chait; Markus Kalkum; Marian Orlowski; Rong Wang

Study of 20 S proteasome activity regulation by histone H3 using multi-mass spectrometric approach

Helena Costa, Andrew Krutchinsky, Brian T. Chait, Markus Kalkum, Marian Orlowski, Rong Wang

Research output: Contribution to conference › Paper › peer-review

Abstract

The effect of histone H3 on proteasome degradation of intact protein was investigated using multi-mass spectrometric method. Degradation of Lysozyme was carried out by incubation of 20 μL of 0.6% solution of lysozyme. Molecular masses of peptides in each fraction were measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometers (MALDI-TOF-MS). The data show that the catalytic activity of proteasome was elevated by H3.

Original language	English
Pages	297-298
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

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title = "Study of 20 S proteasome activity regulation by histone H3 using multi-mass spectrometric approach",

abstract = "The effect of histone H3 on proteasome degradation of intact protein was investigated using multi-mass spectrometric method. Degradation of Lysozyme was carried out by incubation of 20 μL of 0.6% solution of lysozyme. Molecular masses of peptides in each fraction were measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometers (MALDI-TOF-MS). The data show that the catalytic activity of proteasome was elevated by H3.",

author = "Helena Costa and Andrew Krutchinsky and Chait, {Brian T.} and Markus Kalkum and Marian Orlowski and Rong Wang",

year = "2002",

language = "English",

pages = "297--298",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Study of 20 S proteasome activity regulation by histone H3 using multi-mass spectrometric approach

AU - Costa, Helena

AU - Krutchinsky, Andrew

AU - Chait, Brian T.

AU - Kalkum, Markus

AU - Orlowski, Marian

AU - Wang, Rong

PY - 2002

Y1 - 2002

N2 - The effect of histone H3 on proteasome degradation of intact protein was investigated using multi-mass spectrometric method. Degradation of Lysozyme was carried out by incubation of 20 μL of 0.6% solution of lysozyme. Molecular masses of peptides in each fraction were measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometers (MALDI-TOF-MS). The data show that the catalytic activity of proteasome was elevated by H3.

AB - The effect of histone H3 on proteasome degradation of intact protein was investigated using multi-mass spectrometric method. Degradation of Lysozyme was carried out by incubation of 20 μL of 0.6% solution of lysozyme. Molecular masses of peptides in each fraction were measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometers (MALDI-TOF-MS). The data show that the catalytic activity of proteasome was elevated by H3.

UR - http://www.scopus.com/inward/record.url?scp=2442675551&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442675551

SP - 297

EP - 298

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Study of 20 S proteasome activity regulation by histone H3 using multi-mass spectrometric approach

Abstract

Conference

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