TY - JOUR
T1 - Studies on the possible chemical, immunochemical and morphological differences at the cell surfaces of immunosensitive and immunoresistant, moloney virus-induced, lymphoma cell-lines
AU - Das, Hasi R.
AU - Codington, John F.
AU - Lampert, Lorna A.
AU - Maxfield, Mary D.
AU - Jeanloz, Roger W.
AU - Miller, Scott C.
AU - Klein, George
AU - Dalianis, Tina
N1 - Funding Information:
*Dedicated to Professor Elvin A . Kabat . + This work was supported by Public Health Services grants CA-18600 and CA-08418 (to JFC), 5-RO1-CA-14054 (to GK), and DE-04931 (to SCM) ; and by contract DE-AC-02-76EY00119 (to SCM) from the U . S . Department of Energy . This is publication 921 of the Robert W . Lovett Memorial Group for the Study of Diseases Causing Deformities, Harvard Medical School and Massachusetts General Hospital . tPresent address : CSIR Centre for Biochemicals, V . P . Chest Institute Building, University Campus, Delhi-110 007, India, bTo whom communications should be addressed .
PY - 1983/8/16
Y1 - 1983/8/16
N2 - Comparison was made of several cell-surface parameters in the immunosensitive, Moloney virus-induced, mouse lymphoma, YAC, and its immunoresistant variant, YACIR. The characteristics of the two cell lines appeared to be similar by most of the criteria employed. The poly(acrylamide)-gel electrophoresis (with sodium dodecyl sulfate) patterns, after staining with Coomassie Brilliant Blue, of detergent-solubilized materials, appeared to be identical. After elution from a gel-filtration column, no major differences were observed in the protein profiles of material cleaved from viable cells by proteolysis. Scanning and transmission electron microscopy revealed no major differences between the YAC and YACIR cells. The concentration of the lectins, Ricinus communis agglutinin, concanavalin A, wheat-germ agglutinin, and Solanum tuberosum (potato) agglutinin, required to agglutinate viable cells of the two lines were not significantly different. Neither cell was agglutinated by the lectins from Dolichos biflorus or Vicia graminea. Significant differences were, however, observed in the concentrations of lectin from Arachis hypogaea (peanut) needed to agglutinate the two cells. Although similar amounts (184-188 μg/109 cells) of sialic acid were released from viable cells by neuraminidase (V. cholerae), striking differences were observed in the composition of this material: 48% of N-glycolylneuraminic acid for YAC and 15% for YACIR. The remainder was N-acetylneuraminic acid for each cell line.
AB - Comparison was made of several cell-surface parameters in the immunosensitive, Moloney virus-induced, mouse lymphoma, YAC, and its immunoresistant variant, YACIR. The characteristics of the two cell lines appeared to be similar by most of the criteria employed. The poly(acrylamide)-gel electrophoresis (with sodium dodecyl sulfate) patterns, after staining with Coomassie Brilliant Blue, of detergent-solubilized materials, appeared to be identical. After elution from a gel-filtration column, no major differences were observed in the protein profiles of material cleaved from viable cells by proteolysis. Scanning and transmission electron microscopy revealed no major differences between the YAC and YACIR cells. The concentration of the lectins, Ricinus communis agglutinin, concanavalin A, wheat-germ agglutinin, and Solanum tuberosum (potato) agglutinin, required to agglutinate viable cells of the two lines were not significantly different. Neither cell was agglutinated by the lectins from Dolichos biflorus or Vicia graminea. Significant differences were, however, observed in the concentrations of lectin from Arachis hypogaea (peanut) needed to agglutinate the two cells. Although similar amounts (184-188 μg/109 cells) of sialic acid were released from viable cells by neuraminidase (V. cholerae), striking differences were observed in the composition of this material: 48% of N-glycolylneuraminic acid for YAC and 15% for YACIR. The remainder was N-acetylneuraminic acid for each cell line.
UR - http://www.scopus.com/inward/record.url?scp=0021106267&partnerID=8YFLogxK
U2 - 10.1016/0008-6215(83)88024-0
DO - 10.1016/0008-6215(83)88024-0
M3 - Article
C2 - 6627250
AN - SCOPUS:0021106267
SN - 0008-6215
VL - 120
SP - 303
EP - 314
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - C
ER -