Abstract
Experiments have been carried out to determine whether the active uptake of Ca2+ by sporulating B. megaterium cells is driven by the pH gradient across the plasma membrane or by the membrane potential. Results from experiments using the ionophores nigericin and valinomycin which respectively dissipate the ΔpH and the membrane potential, suggest that Ca2+ uptake during sporulation is driven by Δ ψ (the membrane potential). It is further suggested that calcium is transported across the membrane via an antiport system in exchange for one or more protons. Arsenate and an inhibitor reported to be specific for membrane-bound ATPase, efrapeptin, have been used in other experiments to probe the role of ATP generation in calcium transport.
Original language | English |
---|---|
Pages (from-to) | 191-208 |
Number of pages | 18 |
Journal | Microbios |
Volume | 30 |
Issue number | 121-122 |
State | Published - 1981 |
Externally published | Yes |