TY - JOUR
T1 - Studies on bilirubin UDP-glucuronyltransferase
AU - Halac, Elias
AU - Reff, Albert
N1 - Funding Information:
not have been done without the generosityo f Dr. MILTON SALTON and Dr. MENEK GOLDSTEIN in allowing the use of their centrifugesT. his work was partially supported by a grant from the Associationf or the Aid of Crippled Children.
PY - 1967/7/11
Y1 - 1967/7/11
N2 - The capacity of liver fractions to conjugate bilirubin and p-nitrophenol with glucuronic acid is increased several fold by dialysis against alkaline EDTA. UDPglucuronyltransferase (UDP-glucuronate glucuronyltransferase (acceptor-unspecific), EC 2.4.1.17), with activity towards bilirubin and p-nitrophenol, has been solubilized by treating microsomes activated with EDTA and deoxycholate. Kinetic data obtained with activated microsomes, and fractionation and inactivation experiments performed with deoxycholate-solubilized enzyme have yielded evidence in favor of the existence of at least two UDP-glucuronyltransferases. Microsomal pellets were examined by electron microscopy before EDTA, after EDTA, and after EDTA and deoxycholate. The most apparent change that is concomitant with the activation by EDTA is the transformation of rough membranes into smooth membranes.
AB - The capacity of liver fractions to conjugate bilirubin and p-nitrophenol with glucuronic acid is increased several fold by dialysis against alkaline EDTA. UDPglucuronyltransferase (UDP-glucuronate glucuronyltransferase (acceptor-unspecific), EC 2.4.1.17), with activity towards bilirubin and p-nitrophenol, has been solubilized by treating microsomes activated with EDTA and deoxycholate. Kinetic data obtained with activated microsomes, and fractionation and inactivation experiments performed with deoxycholate-solubilized enzyme have yielded evidence in favor of the existence of at least two UDP-glucuronyltransferases. Microsomal pellets were examined by electron microscopy before EDTA, after EDTA, and after EDTA and deoxycholate. The most apparent change that is concomitant with the activation by EDTA is the transformation of rough membranes into smooth membranes.
UR - http://www.scopus.com/inward/record.url?scp=0014200118&partnerID=8YFLogxK
U2 - 10.1016/0005-2744(67)90036-8
DO - 10.1016/0005-2744(67)90036-8
M3 - Article
C2 - 4962257
AN - SCOPUS:0014200118
SN - 0005-2744
VL - 139
SP - 328
EP - 343
JO - BBA - Enzymology
JF - BBA - Enzymology
IS - 2
ER -