Studies in the biotransformation of cortisol to the cortoic acids in man. II. The central role of tetrahydrocortisol and tetrahydrocortisone as intermediates

Barnett Zumoff, Carl Monder, H. Leon Bradlow

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Abstract

The possible role of THF and THE as intermediates in the biotransformation of cortisol to the cortoic acids was studied by giving3H-THF+14C-cortisol tracers and3H-THF+14C cortisol tracers to two subjects each, measuring the3H-THF+14C isotope ratios of the urinary cortoic acid metabolites and relating these ratios to the dose ratio. Isotope ratios substantially higher than the dose ratio indicate that the tetrahydro compound is a better precursor than cortisol, and isotope ratios that are essentially identical to that of urinary THF or THE, respectively, indicate that the tetrahydro compound may be an obligatory intermediate in the cortisol cortoic acids pathway. The isotope ratio data in these studies clearly establish that THF was a preferential precursor of the 11β -hydroxy cortoic acids (cortolic and β-cortolic) and THE was a preferential precursor of the 11-ketonic cortoic acids (cortolonic and β-cortoIonic). Furthermore, the data strongly suggest that THF and THE may be obligatory intermediates in cortoic acid formation.

Original languageEnglish
Pages (from-to)647-650
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume44
Issue number4
DOIs
StatePublished - Apr 1977
Externally publishedYes

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