TY - GEN
T1 - Structure, processing, and polymerization of rainbow trout egg vitelline envelope proteins
AU - Darie, Costel C.
AU - Litscher, Eveline S.
AU - Wassarman, Paul M.
PY - 2008
Y1 - 2008
N2 - Mammalian and non-mammalian eggs are surrounded by a zona pellucida (ZP) and vitelline envelope (VE), respectively. The rainbow trout egg VE consists of three proteins, VEα, VEβ, and VEγ, which are related to mouse egg ZP proteins ZP1, ZP2, and ZP3. Mass spectrometry (MS) has been used extensively to identify the intramolecular disulfide linkages and the cellular site of proteolytic processing of trout VE proteins. Additionally, Blue Native-PAGE (BN-PAGE) has been used to investigate polymerization of purified trout VE proteins under non-denaturing conditions. Results of these experiments reveal that, despite ∼400 million years separating the appearance of trout and mice, and the change from external to internal fertilization and development, VE and ZP proteins have a great deal in common.
AB - Mammalian and non-mammalian eggs are surrounded by a zona pellucida (ZP) and vitelline envelope (VE), respectively. The rainbow trout egg VE consists of three proteins, VEα, VEβ, and VEγ, which are related to mouse egg ZP proteins ZP1, ZP2, and ZP3. Mass spectrometry (MS) has been used extensively to identify the intramolecular disulfide linkages and the cellular site of proteolytic processing of trout VE proteins. Additionally, Blue Native-PAGE (BN-PAGE) has been used to investigate polymerization of purified trout VE proteins under non-denaturing conditions. Results of these experiments reveal that, despite ∼400 million years separating the appearance of trout and mice, and the change from external to internal fertilization and development, VE and ZP proteins have a great deal in common.
UR - http://www.scopus.com/inward/record.url?scp=69749100167&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:69749100167
SN - 9781402088100
T3 - NATO Science for Peace and Security Series A: Chemistry and Biology
SP - 23
EP - 36
BT - Applications of Mass Spectrometry in Life Safety
A2 - Popescu, Crisan
A2 - Zamfir, Alina
A2 - Dinca, Nicolae
ER -