Structure of nucleoside diphosphate kinase from pacific shrimp (Litopenaeus vannamei) in binary complexes with purine and pyrimidine nucleoside diphosphates

Alonso A. López-Zavala, Idania E. Quintero-Reyes, Jesús S. Carrasco-Miranda, Vivian Stojanoff, Andrzej Weichsel, Enrique Rudiño-Piñera, Rogerio R. Sotelo-Mundo

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Abstract

Nucleoside diphosphate kinase (NDK; EC 2.7.4.6) is an enzyme that catalyzes the third phosphorylation of nucleoside diphosphates, leading to nucleoside triphosphates for DNA replication. Expression of the NDK from Litopenaeus vannamei (LvNDK) is known to be regulated under viral infection. Also, as determined by isothermal titration calorimetry, LvNDK binds both purine and pyrimidine deoxynucleoside diphosphates with high binding affinity for dGDP and dADP and with no heat of binding interaction for dCDP [Quintero-Reyes et al. (2012), J. Bioenerg. Biomembr. 44, 325-331]. In order to investigate the differences in selectivity, LvNDK was crystallized as binary complexes with both acceptor (dADP and dCDP) and donor (ADP) phosphate-group nucleoside diphosphate substrates and their structures were determined. The three structures with purine or pyrimidine nucleotide ligands are all hexameric. Also, the binding of deoxy or ribonucleotides is similar, as in the former a water molecule replaces the hydrogen bond made by Lys11 to the 2′-hydroxyl group of the ribose moiety. This allows Lys11 to maintain a catalytically favourable conformation independently of the kind of sugar found in the nucleotide. Because of this, shrimp NDK may phosphorylate nucleotide analogues to inhibit the viral infections that attack this organism.

Original languageEnglish
Pages (from-to)1150-1154
Number of pages5
JournalActa Crystallographica Section F:Structural Biology Communications
Volume70
DOIs
StatePublished - 1 Sep 2014
Externally publishedYes

Keywords

  • Litopenaeus vannamei
  • deoxyadenine binary complex
  • nucleoside diphosphate kinase
  • shrimp

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