Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis

Jing Hou; Kamila Wojciechowska; Heping Zheng; Maksymilian Chruszcz; David R. Cooper; Marcin Cymborowski; Tatiana Skarina; Elena Gordon; Haibin Luo; Alexei Savchenko; Wladek Minor

doi:10.1107/S1744309112017939

Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis

Jing Hou, Kamila Wojciechowska, Heping Zheng, Maksymilian Chruszcz, David R. Cooper, Marcin Cymborowski, Tatiana Skarina, Elena Gordon, Haibin Luo, Alexei Savchenko, Wladek Minor

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The crystal structure of a short-chain dehydrogenase/reductase from Bacillus anthracis strain Ames Ancestor complexed with NADP has been determined and refined to 1.87 Å resolution. The structure of the enzyme consists of a Rossmann fold composed of seven parallel Β-strands sandwiched by three - helices on each side. An NADP molecule from an endogenous source is bound in the conserved binding pocket in the syn conformation. The loop region responsible for binding another substrate forms two perpendicular short helices connected by a sharp turn.

Original language	English
Pages (from-to)	632-637
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	68
Issue number	6
DOIs	https://doi.org/10.1107/S1744309112017939
State	Published - May 2012
Externally published	Yes

Keywords

Bacillus anthracis
NADP
short-chain dehydrogenases/reductases

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10.1107/S1744309112017939

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Hou, J., Wojciechowska, K., Zheng, H., Chruszcz, M., Cooper, D. R., Cymborowski, M., Skarina, T., Gordon, E., Luo, H., Savchenko, A., & Minor, W. (2012). Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 68(6), 632-637. https://doi.org/10.1107/S1744309112017939

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title = "Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis",

abstract = "The crystal structure of a short-chain dehydrogenase/reductase from Bacillus anthracis strain Ames Ancestor complexed with NADP has been determined and refined to 1.87 {\AA} resolution. The structure of the enzyme consists of a Rossmann fold composed of seven parallel Β-strands sandwiched by three - helices on each side. An NADP molecule from an endogenous source is bound in the conserved binding pocket in the syn conformation. The loop region responsible for binding another substrate forms two perpendicular short helices connected by a sharp turn.",

keywords = "Bacillus anthracis, NADP, short-chain dehydrogenases/reductases",

author = "Jing Hou and Kamila Wojciechowska and Heping Zheng and Maksymilian Chruszcz and Cooper, {David R.} and Marcin Cymborowski and Tatiana Skarina and Elena Gordon and Haibin Luo and Alexei Savchenko and Wladek Minor",

year = "2012",

month = may,

doi = "10.1107/S1744309112017939",

language = "English",

volume = "68",

pages = "632--637",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "6",

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Hou, J, Wojciechowska, K, Zheng, H, Chruszcz, M, Cooper, DR, Cymborowski, M, Skarina, T, Gordon, E, Luo, H, Savchenko, A & Minor, W 2012, 'Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 68, no. 6, pp. 632-637. https://doi.org/10.1107/S1744309112017939

TY - JOUR

T1 - Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis

AU - Hou, Jing

AU - Wojciechowska, Kamila

AU - Zheng, Heping

AU - Chruszcz, Maksymilian

AU - Cooper, David R.

AU - Cymborowski, Marcin

AU - Skarina, Tatiana

AU - Gordon, Elena

AU - Luo, Haibin

AU - Savchenko, Alexei

AU - Minor, Wladek

PY - 2012/5

Y1 - 2012/5

N2 - The crystal structure of a short-chain dehydrogenase/reductase from Bacillus anthracis strain Ames Ancestor complexed with NADP has been determined and refined to 1.87 Å resolution. The structure of the enzyme consists of a Rossmann fold composed of seven parallel Β-strands sandwiched by three - helices on each side. An NADP molecule from an endogenous source is bound in the conserved binding pocket in the syn conformation. The loop region responsible for binding another substrate forms two perpendicular short helices connected by a sharp turn.

AB - The crystal structure of a short-chain dehydrogenase/reductase from Bacillus anthracis strain Ames Ancestor complexed with NADP has been determined and refined to 1.87 Å resolution. The structure of the enzyme consists of a Rossmann fold composed of seven parallel Β-strands sandwiched by three - helices on each side. An NADP molecule from an endogenous source is bound in the conserved binding pocket in the syn conformation. The loop region responsible for binding another substrate forms two perpendicular short helices connected by a sharp turn.

KW - Bacillus anthracis

KW - NADP

KW - short-chain dehydrogenases/reductases

UR - http://www.scopus.com/inward/record.url?scp=84862239275&partnerID=8YFLogxK

U2 - 10.1107/S1744309112017939

DO - 10.1107/S1744309112017939

M3 - Article

C2 - 22684058

AN - SCOPUS:84862239275

SN - 1744-3091

VL - 68

SP - 632

EP - 637

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 6

ER -

Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis

Abstract

Keywords

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