Structure, expression pattern and chromosomal localization of the rice Osgrp-2 gene

Zongzhi Liu, Jianlong Wang, Qun Wang, Xun Huang, Weihui Xu, Lihuang Zhu, Ping He, Rongxiang Fang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Glycine-rich proteins (GRPs) belong to a kind of important structural proteins of plant cell walls. The expression of GRP genes is regulated spatially and developmentally as well as by various environmental stresses, thus providing a good model for the study of plant gene expression. We obtained the genomic sequence of a new GRP gene (Osgrp-2) from a rice genomic library. The transcription start site of Osgrp-2 was determined by 5′-rapid amplification of cDNA ends (RACE) and a 2.4-kb promoter sequence was thus delimited. The spatial and developmental expression pattern as well as the wound-inducible character of Osgrp-2 in rice plants was analyzed in detail. Furthermore, the gene was mapped onto rice chromosome 10 by analysis of restriction fragment length polymorphism (RFLP).

Original languageEnglish
Pages (from-to)584-594
Number of pages11
JournalScience in China, Series C: Life Sciences
Volume46
Issue number6
DOIs
StatePublished - 2003
Externally publishedYes

Keywords

  • Chromosomal localization
  • Expression pattern
  • GRP
  • Gene structure
  • Oryza sativa

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