Structural, functional, and inhibition studies of a Gcn5-related N-acetyltransferase (GNAT) superfamily protein PA4794: A new C-terminal lysine protein acetyltransferase from pseudomonas aeruginosa

Karolina A. Majorek, Misty L. Kuhn, Maksymilian Chruszcz, Wayne F. Anderson, Wladek Minor

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Background: Gcn5-related N-acetyltransferases (GNATs) are involved in small molecule and protein acetylation in all organisms. Results: Crystallographic and biochemical characterization of PA4794 is shown, including identification of substrates and inhibitors. Conclusion: PA4794 is a new bacterial C-terminal lysine protein acetyltransferase inhibited by cephalosporins. Significance: PA4794 is the first identified acetyltransferase specific for C-terminal lysine; identified interactions with cephalosporins may be of clinical relevance.

Original languageEnglish
Pages (from-to)30223-30235
Number of pages13
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
StatePublished - 18 Oct 2013
Externally publishedYes

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