Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus

Pengfei He; Anqiang Zhang; Saijing Zhou; Fuming Zhang; Robert J. Linhardt; Peilong Sun

doi:10.1016/j.carres.2016.08.006

Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus

Pengfei He
, Anqiang Zhang
, Saijing Zhou
, Fuming Zhang
, Robert J. Linhardt
, Peilong Sun

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A water-soluble polysaccharide containing 3-O-methyl galactose (PCP60W) was isolated from fruiting bodies of Pleurotus citrinopileatus and purified by anion-exchange and gel column chromatography. This polysaccharide has an average molecular weight of 2.74 × 10⁴ Da and its structure was elucidated using monosaccharide composition and methylation analysis combined with one- and two-dimensional (COSY, TOCSY, NOESY, HMQC and HMBC) NMR spectroscopy. PCP60W was shown to be a linear partially 3-O-methylated α-galactopyranan comprised of 6-linked galactose, 6-linked 3-O-methyl galactose and 4-linked glucose in a ratio of 3.0:1.0:0.6. This work provides additional evidence for the view that 3-O-methyl galactose is common to the genus Pleurotus.

Original language	English
Pages (from-to)	72-76
Number of pages	5
Journal	Carbohydrate Research
Volume	434
DOIs	https://doi.org/10.1016/j.carres.2016.08.006
State	Published - 2016
Externally published	Yes

Keywords

3-O-methylated galactopyranose
Edible mushrooms
Pleurotus citrinopileatus
Polysaccharide
Structure

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10.1016/j.carres.2016.08.006

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@article{a4c27acadbfe49eba0cc0307ed1dcca5,

title = "Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus",

abstract = "A water-soluble polysaccharide containing 3-O-methyl galactose (PCP60W) was isolated from fruiting bodies of Pleurotus citrinopileatus and purified by anion-exchange and gel column chromatography. This polysaccharide has an average molecular weight of 2.74 × 104 Da and its structure was elucidated using monosaccharide composition and methylation analysis combined with one- and two-dimensional (COSY, TOCSY, NOESY, HMQC and HMBC) NMR spectroscopy. PCP60W was shown to be a linear partially 3-O-methylated α-galactopyranan comprised of 6-linked galactose, 6-linked 3-O-methyl galactose and 4-linked glucose in a ratio of 3.0:1.0:0.6. This work provides additional evidence for the view that 3-O-methyl galactose is common to the genus Pleurotus.",

keywords = "3-O-methylated galactopyranose, Edible mushrooms, Pleurotus citrinopileatus, Polysaccharide, Structure",

author = "Pengfei He and Anqiang Zhang and Saijing Zhou and Fuming Zhang and Linhardt, \{Robert J.\} and Peilong Sun",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

year = "2016",

doi = "10.1016/j.carres.2016.08.006",

language = "English",

volume = "434",

pages = "72--76",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus

AU - He, Pengfei

AU - Zhang, Anqiang

AU - Zhou, Saijing

AU - Zhang, Fuming

AU - Linhardt, Robert J.

AU - Sun, Peilong

PY - 2016

Y1 - 2016

N2 - A water-soluble polysaccharide containing 3-O-methyl galactose (PCP60W) was isolated from fruiting bodies of Pleurotus citrinopileatus and purified by anion-exchange and gel column chromatography. This polysaccharide has an average molecular weight of 2.74 × 104 Da and its structure was elucidated using monosaccharide composition and methylation analysis combined with one- and two-dimensional (COSY, TOCSY, NOESY, HMQC and HMBC) NMR spectroscopy. PCP60W was shown to be a linear partially 3-O-methylated α-galactopyranan comprised of 6-linked galactose, 6-linked 3-O-methyl galactose and 4-linked glucose in a ratio of 3.0:1.0:0.6. This work provides additional evidence for the view that 3-O-methyl galactose is common to the genus Pleurotus.

AB - A water-soluble polysaccharide containing 3-O-methyl galactose (PCP60W) was isolated from fruiting bodies of Pleurotus citrinopileatus and purified by anion-exchange and gel column chromatography. This polysaccharide has an average molecular weight of 2.74 × 104 Da and its structure was elucidated using monosaccharide composition and methylation analysis combined with one- and two-dimensional (COSY, TOCSY, NOESY, HMQC and HMBC) NMR spectroscopy. PCP60W was shown to be a linear partially 3-O-methylated α-galactopyranan comprised of 6-linked galactose, 6-linked 3-O-methyl galactose and 4-linked glucose in a ratio of 3.0:1.0:0.6. This work provides additional evidence for the view that 3-O-methyl galactose is common to the genus Pleurotus.

KW - 3-O-methylated galactopyranose

KW - Edible mushrooms

KW - Pleurotus citrinopileatus

KW - Polysaccharide

KW - Structure

UR - https://www.scopus.com/pages/publications/84985937221

U2 - 10.1016/j.carres.2016.08.006

DO - 10.1016/j.carres.2016.08.006

M3 - Article

C2 - 27599128

AN - SCOPUS:84985937221

SN - 0008-6215

VL - 434

SP - 72

EP - 76

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus

Abstract

Keywords

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