Structural and functional insight into the universal stress protein family

Karolina L. Tkaczuk, Igor A. Shumilin, Maksymilian Chruszcz, Elena Evdokimova, Alexei Savchenko, Wladek Minor

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

We present the crystal structures of two universal stress proteins (USP) from Archaeoglobus fulgidus and Nitrosomonas europaea in both apo- and ligand-bound forms. This work is the first complete synthesis of the structural properties of 26 USP available in the Protein Data Bank, over 75% of which were determined by structure genomics centers with no additional information provided. The results of bioinformatic analyses of all available USP structures and their sequence homologs revealed that these two new USP structures share overall structural similarity with structures of USPs previously determined. Clustering and cladogram analyses, however, show how they diverge from other members of the USP superfamily and show greater similarity to USPs from organisms inhabiting extreme environments. We compared them with other archaeal and bacterial USPs and discuss their similarities and differences in context of structure, sequential motifs, and potential function. We also attempted to group all analyzed USPs into families, so that assignment of the potential function to those with no experimental data available would be possible by extrapolation.

Original languageEnglish
Pages (from-to)434-449
Number of pages16
JournalEvolutionary Applications
Volume6
Issue number3
DOIs
StatePublished - Apr 2013
Externally publishedYes

Keywords

  • Archaeoglobus fulgidus
  • Crystal structures
  • Nitrosomonas europaea
  • Pathogens
  • Sequence analyses
  • Structural comparison
  • Structural genomics
  • Universal stress protein

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