Structural analysis of the polysaccharide from the lipopolysaccharide of Porphyromonas gingivalis strain W50

Nikolay Paramonov, David Bailey, Minnie Rangarajan, Ahmed Hashim, Geoff Kelly, Michael A. Curtis, Elizabeth F. Hounsell

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

The lipopolysaccharide (LPS) of Porphyromonas gingivalis is an important pro-inflammatory molecule in periodontal disease and a significant target of the host's specific immune response. In addition, we recently demonstrated using monoclonal antibodies that the Arg-gingipains of P. gingivalis are post-translationally modified with glycan chains that are immunologically related to an LPS preparation from this organism. In the present investigation, we determined the structure of the O-polysaccharide of P. gingivalis W50 that was fully characterized on the basis of 1D and 2D NMR (DQF-COSY, TOCSY, NOESY, ROESY, 1H-13C HSQC and 1H-31P HXTOCSY) and GC-MS data. These data allowed us to conclude that the O-polysaccharide is built up of the tetrasaccharide repeating sequence: →6)-α-D-Glcp-(1→4)-α-L-Rhap-(1→3)-β-D- GalNAc-(1→3)-α-D-Galp-(1→ and carries a monophospho-ethanolamine residue at position C-2 of the α-rhamnose residue in a nonstoichiometric (approximately 60%) amount. These data indicate that the O-polysaccharide of P. gingivalis LPS is composed of an unusually modified tetrasaccharide repeating unit.

Original languageEnglish
Pages (from-to)4698-4707
Number of pages10
JournalEuropean Journal of Biochemistry
Volume268
Issue number17
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Arg-gingipain
  • Lipopolysaccharide
  • NMR
  • O-polysaccharide
  • P. gingivalis

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