Stimulation of vgf gene expression by NGF is mediated through multiple signal transduction pathways involving protein phosphorylation

Stephen R.J. Salton; Cinzia Volonté; Gabriella D'Arcangelo

doi:10.1016/0014-5793(95)00086-O

Stimulation of vgf gene expression by NGF is mediated through multiple signal transduction pathways involving protein phosphorylation

Stephen R.J. Salton
, Cinzia Volonté
, Gabriella D'Arcangelo

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The vgf gene encodes one of the most rapidly induced neuronal mRNAs identified in NGF-treated PC12 cells. Maximal inhibition of VGF mRNA induction was achieved using K-252a, an inhibitor of the NGF-receptor Trk tyrosine kinase, and by mutating both Y490 (SHC association site) and Y785 (PLC-γ1 association site) of Trk. Inhibitors of the NGF-activated protein kinase N (PKN) were found to partially and in some cases transiently block VGF induction by NGF while in PKA-deficient PC12 cells, VGF induction by NGF was comparable to that observed in parental PC12 cells. The binding of NGF to Trk therefore activates redundant signal transduction pathways which converge to regulate vgf gene expression.

Original language	English
Pages (from-to)	106-110
Number of pages	5
Journal	FEBS Letters
Volume	360
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)00086-O
State	Published - 27 Feb 1995

Keywords

NGF
PC12 cell
Protein kinase N
Protein phosphorylation
Trk
VGF

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10.1016/0014-5793(95)00086-O

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title = "Stimulation of vgf gene expression by NGF is mediated through multiple signal transduction pathways involving protein phosphorylation",

abstract = "The vgf gene encodes one of the most rapidly induced neuronal mRNAs identified in NGF-treated PC12 cells. Maximal inhibition of VGF mRNA induction was achieved using K-252a, an inhibitor of the NGF-receptor Trk tyrosine kinase, and by mutating both Y490 (SHC association site) and Y785 (PLC-γ1 association site) of Trk. Inhibitors of the NGF-activated protein kinase N (PKN) were found to partially and in some cases transiently block VGF induction by NGF while in PKA-deficient PC12 cells, VGF induction by NGF was comparable to that observed in parental PC12 cells. The binding of NGF to Trk therefore activates redundant signal transduction pathways which converge to regulate vgf gene expression.",

keywords = "NGF, PC12 cell, Protein kinase N, Protein phosphorylation, Trk, VGF",

author = "Salton, \{Stephen R.J.\} and Cinzia Volont{\'e} and Gabriella D'Arcangelo",

year = "1995",

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doi = "10.1016/0014-5793(95)00086-O",

language = "English",

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TY - JOUR

T1 - Stimulation of vgf gene expression by NGF is mediated through multiple signal transduction pathways involving protein phosphorylation

AU - Salton, Stephen R.J.

AU - Volonté, Cinzia

AU - D'Arcangelo, Gabriella

PY - 1995/2/27

Y1 - 1995/2/27

N2 - The vgf gene encodes one of the most rapidly induced neuronal mRNAs identified in NGF-treated PC12 cells. Maximal inhibition of VGF mRNA induction was achieved using K-252a, an inhibitor of the NGF-receptor Trk tyrosine kinase, and by mutating both Y490 (SHC association site) and Y785 (PLC-γ1 association site) of Trk. Inhibitors of the NGF-activated protein kinase N (PKN) were found to partially and in some cases transiently block VGF induction by NGF while in PKA-deficient PC12 cells, VGF induction by NGF was comparable to that observed in parental PC12 cells. The binding of NGF to Trk therefore activates redundant signal transduction pathways which converge to regulate vgf gene expression.

AB - The vgf gene encodes one of the most rapidly induced neuronal mRNAs identified in NGF-treated PC12 cells. Maximal inhibition of VGF mRNA induction was achieved using K-252a, an inhibitor of the NGF-receptor Trk tyrosine kinase, and by mutating both Y490 (SHC association site) and Y785 (PLC-γ1 association site) of Trk. Inhibitors of the NGF-activated protein kinase N (PKN) were found to partially and in some cases transiently block VGF induction by NGF while in PKA-deficient PC12 cells, VGF induction by NGF was comparable to that observed in parental PC12 cells. The binding of NGF to Trk therefore activates redundant signal transduction pathways which converge to regulate vgf gene expression.

KW - NGF

KW - PC12 cell

KW - Protein kinase N

KW - Protein phosphorylation

KW - Trk

KW - VGF

UR - https://www.scopus.com/pages/publications/0028927421

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DO - 10.1016/0014-5793(95)00086-O

M3 - Article

C2 - 7875312

AN - SCOPUS:0028927421

SN - 0014-5793

VL - 360

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EP - 110

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Stimulation of vgf gene expression by NGF is mediated through multiple signal transduction pathways involving protein phosphorylation

Abstract

Keywords

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