Stimulation of type IV collagenase expression by linoleic acid in a metastatic human breast cancer cell line

Xin Hua Liu; David P. Rose

doi:10.1016/0304-3835(94)90136-8

Stimulation of type IV collagenase expression by linoleic acid in a metastatic human breast cancer cell line

Xin Hua Liu, David P. Rose

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Linoleic acid (LA), an omega-6 fatty acid, enhanced the appearance of type IV collagenase activity in culture medium conditioned by the metastatic MDA-MB-435 human breast cancer cell line; this effect was maximal with 0.75 μg/ml LA. Zymography showed an increase in the gelatinolytic 92 kDa metalloproteinase, a form associated with the metastatic phenotype, during culture in the presence of 0.75 μg/ml LA. Indomethacin, 20 μg/ml, completely suppressed the stimulation of collagenase by LA, suggesting a role for the eicosanoids. The tumor cells expressed mRNA for both the 72 and 92 kDa isoforms of type IV collagenase. Basal levels of the 92 kDa mRNA were much higher; both were up-regulated by LA despite the absence of detectable 72 kDa activity in conditioned medium.

Original language	English
Pages (from-to)	71-77
Number of pages	7
Journal	Cancer Letters
Volume	76
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(94)90136-8
State	Published - 15 Jan 1994
Externally published	Yes

Keywords

Breast cancer
Collagenase
Linoleic acid

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10.1016/0304-3835(94)90136-8

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title = "Stimulation of type IV collagenase expression by linoleic acid in a metastatic human breast cancer cell line",

abstract = "Linoleic acid (LA), an omega-6 fatty acid, enhanced the appearance of type IV collagenase activity in culture medium conditioned by the metastatic MDA-MB-435 human breast cancer cell line; this effect was maximal with 0.75 μg/ml LA. Zymography showed an increase in the gelatinolytic 92 kDa metalloproteinase, a form associated with the metastatic phenotype, during culture in the presence of 0.75 μg/ml LA. Indomethacin, 20 μg/ml, completely suppressed the stimulation of collagenase by LA, suggesting a role for the eicosanoids. The tumor cells expressed mRNA for both the 72 and 92 kDa isoforms of type IV collagenase. Basal levels of the 92 kDa mRNA were much higher; both were up-regulated by LA despite the absence of detectable 72 kDa activity in conditioned medium.",

keywords = "Breast cancer, Collagenase, Linoleic acid",

author = "Liu, {Xin Hua} and Rose, {David P.}",

note = "Funding Information: We thank Arlene Banow for assistance in preparing the manuscript. This study was supported by Grant No. CN-100 from the American Cancer Society.",

year = "1994",

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T1 - Stimulation of type IV collagenase expression by linoleic acid in a metastatic human breast cancer cell line

AU - Liu, Xin Hua

AU - Rose, David P.

N1 - Funding Information: We thank Arlene Banow for assistance in preparing the manuscript. This study was supported by Grant No. CN-100 from the American Cancer Society.

PY - 1994/1/15

Y1 - 1994/1/15

N2 - Linoleic acid (LA), an omega-6 fatty acid, enhanced the appearance of type IV collagenase activity in culture medium conditioned by the metastatic MDA-MB-435 human breast cancer cell line; this effect was maximal with 0.75 μg/ml LA. Zymography showed an increase in the gelatinolytic 92 kDa metalloproteinase, a form associated with the metastatic phenotype, during culture in the presence of 0.75 μg/ml LA. Indomethacin, 20 μg/ml, completely suppressed the stimulation of collagenase by LA, suggesting a role for the eicosanoids. The tumor cells expressed mRNA for both the 72 and 92 kDa isoforms of type IV collagenase. Basal levels of the 92 kDa mRNA were much higher; both were up-regulated by LA despite the absence of detectable 72 kDa activity in conditioned medium.

AB - Linoleic acid (LA), an omega-6 fatty acid, enhanced the appearance of type IV collagenase activity in culture medium conditioned by the metastatic MDA-MB-435 human breast cancer cell line; this effect was maximal with 0.75 μg/ml LA. Zymography showed an increase in the gelatinolytic 92 kDa metalloproteinase, a form associated with the metastatic phenotype, during culture in the presence of 0.75 μg/ml LA. Indomethacin, 20 μg/ml, completely suppressed the stimulation of collagenase by LA, suggesting a role for the eicosanoids. The tumor cells expressed mRNA for both the 72 and 92 kDa isoforms of type IV collagenase. Basal levels of the 92 kDa mRNA were much higher; both were up-regulated by LA despite the absence of detectable 72 kDa activity in conditioned medium.

KW - Breast cancer

KW - Collagenase

KW - Linoleic acid

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Stimulation of type IV collagenase expression by linoleic acid in a metastatic human breast cancer cell line

Abstract

Keywords

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