TY - JOUR
T1 - Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins [16]
AU - Hildebrandt, John D.
AU - Sekura, Ronald D.
AU - Codina, Juan
AU - Iyengar, Ravi
AU - Manclark, Charles R.
AU - Birnbaumer, Lutz
PY - 1983
Y1 - 1983
N2 - Adenylyl cyclases are under positive and negative control by guanine nucleotides and hormones1-3. Stimulatory responses are mediated by a guanine nucleotide- and Mg-binding regulatory component4-6 (N s), a protein that has been purified to homogeneity7-9. Inhibitory responses have been hypothesized1-3,10,11 to be mediated by an analogous regulatory component (Ni) distinct from N s, but definitive proof for this is lacking and these effects may result from modulation of Ns activity. Recently, Bordetella pertussis toxin has been shown12 to ADP-ribosylate a peptide that is not part of N s, and this coincides with attenuation of hormonal inhibition of adenylyl cyclase12-17. We show here that cyc- S49 cells contain a substrate for ADP-ribosylation by pertussis toxin and that the toxin alters GTP dependent inhibition of cyc- adenyl cyclase activity 18. As cyc- S49 cells do not contain Ns by several criteria5,19-24, we conclude that Ni is a distinct and separate regulatory component of adenylyl cyclase.
AB - Adenylyl cyclases are under positive and negative control by guanine nucleotides and hormones1-3. Stimulatory responses are mediated by a guanine nucleotide- and Mg-binding regulatory component4-6 (N s), a protein that has been purified to homogeneity7-9. Inhibitory responses have been hypothesized1-3,10,11 to be mediated by an analogous regulatory component (Ni) distinct from N s, but definitive proof for this is lacking and these effects may result from modulation of Ns activity. Recently, Bordetella pertussis toxin has been shown12 to ADP-ribosylate a peptide that is not part of N s, and this coincides with attenuation of hormonal inhibition of adenylyl cyclase12-17. We show here that cyc- S49 cells contain a substrate for ADP-ribosylation by pertussis toxin and that the toxin alters GTP dependent inhibition of cyc- adenyl cyclase activity 18. As cyc- S49 cells do not contain Ns by several criteria5,19-24, we conclude that Ni is a distinct and separate regulatory component of adenylyl cyclase.
UR - https://www.scopus.com/pages/publications/0020570477
U2 - 10.1038/302706a0
DO - 10.1038/302706a0
M3 - Letter
C2 - 6300694
AN - SCOPUS:0020570477
SN - 0028-0836
VL - 302
SP - 706
EP - 709
JO - Nature
JF - Nature
IS - 5910
ER -