TY - JOUR
T1 - Static magnetic field modulation of myosin phosphorylation
T2 - Calcium dependence in two enzyme preparations
AU - Markov, M. S.
AU - Pilla, A. A.
PY - 1994/11
Y1 - 1994/11
N2 - The effects of weak, environmental range, static (d.c.) magnetic fields on myosin light chain phosphorylation in two enzyme preparations are reported. Specifically, the proposal that sensitivity to microtesla-level magnetic fields may be related to Ca2+ ion binding is examined using both myosin light chain kinase and protein kinase C assays. The electromagnetic field exposure system allowed spatial control of the applied static magnetic field in the 0.1-200 μT range in the absence of a.c. components above ± 0.1 μT. The results showed that phosphorylation strongly depends on calcium ion concentration in the reaction mixture. Of interest is the observation that the magnetic field effect is maximal at low Ca2+ concentration for the calmodulin assay, whereas high Ca2+ concentration is required for the protein kinase C assay.
AB - The effects of weak, environmental range, static (d.c.) magnetic fields on myosin light chain phosphorylation in two enzyme preparations are reported. Specifically, the proposal that sensitivity to microtesla-level magnetic fields may be related to Ca2+ ion binding is examined using both myosin light chain kinase and protein kinase C assays. The electromagnetic field exposure system allowed spatial control of the applied static magnetic field in the 0.1-200 μT range in the absence of a.c. components above ± 0.1 μT. The results showed that phosphorylation strongly depends on calcium ion concentration in the reaction mixture. Of interest is the observation that the magnetic field effect is maximal at low Ca2+ concentration for the calmodulin assay, whereas high Ca2+ concentration is required for the protein kinase C assay.
KW - Enzymes
KW - Myosin
KW - Phosphorylation
KW - Static magnetic fields
UR - http://www.scopus.com/inward/record.url?scp=0006426429&partnerID=8YFLogxK
U2 - 10.1016/0302-4598(94)87012-8
DO - 10.1016/0302-4598(94)87012-8
M3 - Article
AN - SCOPUS:0006426429
SN - 0302-4598
VL - 35
SP - 57
EP - 61
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
IS - 1-2
ER -